Fabrication of long poly(dimethyl siloxane) nanochannels by replicating protein deposit from confined solution evaporation |
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| Authors: | Kuo-Feng Lo Yi-Je Juang |
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| Institution: | 1.Department of Chemical Engineering, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan;2.Center for Micro/Nano Science and Technology, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan;3.Research Center for Energy Technology and Strategy, National Cheng Kung University, No. 1 University Road, Tainan 70101, Taiwan |
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| Abstract: | A relatively simple, inexpensive and reliable technique was developed to fabricate an array of nanochannels. Moreover, the nanochannels are directly integrated to microchannels as a whole, which facilitates solution loading from the millimeter-scaled loading reservoirs into the nanochannels. It is found that continuous bovine serum albumin (BSA) line structures with triangle-like cross section at nanoscale can be obtained by evaporation of BSA solution with concentration between 0.5 wt. % and 1 wt. % inside the microchannels. The poly(dimethyl siloxane) nanochannels were replicated from these line structures, followed by sealing with the glass slide. The DNA molecules can be stretched inside the nanochannels as fabricated. |
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