| 商陆寡肽转运蛋白基因PaOPT的cDNA全序列克隆及表达分析 |
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| 引用本文： | 金力,郑宏春,柴团耀.商陆寡肽转运蛋白基因PaOPT的cDNA全序列克隆及表达分析[J].中国科学院研究生院学报,2012,29(3):316-323. |
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| 作者姓名： | 金力 郑宏春 柴团耀 |
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| 作者单位： | 1. 中国科学院研究生院生命科学学院,北京,100049
2. 中国科学院研究生院生命科学学院,北京100049;陕西教育学院生物科学与技术系,西安710100 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，转基因生物新品种培育科技重大专项 |
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| 摘 要： | 从垂序商陆(Phytolacca americana)的重金属镉胁迫下商陆幼苗叶片的正反向抑制性消减杂交文库(SSH文库)中,获得商陆寡肽转运蛋白基因的EST序列.利用RACE的方法克隆到该基因的全序列cDNA(PaOPT,GenBank注册HQ647015).PaOPT基因在进化中的地位分析表明:它可能属于OPT3基因家族.利用实时荧光定量PCR的方法,分析PaOPT的组织表达特异性以及商陆种子成熟及萌发过程的表达模式.结果表明,该基因是一个在种子中特异性表达的基因,预测它对植物的生长发育有着重要的作用.
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| 关 键 词： | 商陆 寡肽转运蛋白 表达模式 |
Cloning and expression of PaOPT in Phytolacca americana |
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| JIN Li
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ZHENG Hong-Chun
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CHAI Tuan-Yao.Cloning and expression of PaOPT in Phytolacca americana[J].Journal of the Graduate School of the Chinese Academy of Sciences,2012,29(3):316-323. |
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| Authors: | JIN Li ZHENG Hong-Chun CHAI Tuan-Yao |
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| Institution: | 1(1 College of Life Science,Graduate University,Chinese Academy of Sciences,Beijing 100049,China; 2 Department of Life Science and Technology,Shaanxi Institute of Education,Xi’an 710100,China) |
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| Abstract: | An oligopeptide transporter gene was isolated from Cd-treated leaf tissue of Phytolacca americana by SSH method,designated by PaOPT(GenBank accession No.HQ647015).The full length of this gene has been cloned by race-PCR technique.Moreover,the evolution of PaOPT in OPT family and the expression profiles during seed germination period was investigated.Our study indicates that PaOPT belongs to the OPT3 family.The results of real-time quantitative PCR show that PaOPT may be a seed-specific expression gene.Further investigation of the function and localization of PaOPT will be favorable for understanding of plant growth and development. |
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| Keywords: | Phytolacca Americana PaOPT expression |
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