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API细菌鉴定系统应用于环境微生物学实验
引用本文:王士芬,乐毅全,施鼎方,唐贤春,张铮,周莉,沈雁文,张琼.API细菌鉴定系统应用于环境微生物学实验[J].实验室研究与探索,2007,26(10):17-18,100.
作者姓名:王士芬  乐毅全  施鼎方  唐贤春  张铮  周莉  沈雁文  张琼
作者单位:同济大学,环境科学与工程学院,上海,200092
摘    要:API 20E细菌鉴定系统(API 20E System)采用多个生物化学指标和数据库对肠杆菌科和其它革兰氏阴性杆菌进行鉴定。从环境中分离到的细菌经革兰氏染色和氧化酶试验后,若均呈阴性结果,就可选用API 20E细菌鉴定系统鉴定细菌。将本方法应用于学生教学实验,使学生的能力得到提高,取得很好的效果。

关 键 词:细菌鉴定系统  菌种鉴定  环境工程微生物学实验
文章编号:1006-7167(2007)10-0017-03
收稿时间:2006-12-01
修稿时间:2006-12-01

The Application of API System to Environmental Microbiological Experiment
WANG Shi-fen,LE Yi-quan,SHI Ding-fang,TANG Xian-chun,ZHANG Zheng,ZHOU Li,SHEN Yan-wen,ZHANG Qiong.The Application of API System to Environmental Microbiological Experiment[J].Laboratory Research and Exploration,2007,26(10):17-18,100.
Authors:WANG Shi-fen  LE Yi-quan  SHI Ding-fang  TANG Xian-chun  ZHANG Zheng  ZHOU Li  SHEN Yan-wen  ZHANG Qiong
Institution:School of Environmental Science and Eng. ,Tongji Univ. , Shanghai 200092, China
Abstract:API 20E system is a standardized system for enterobacteriaceae and other Gram-negative rods which uses the miniaturized biochemical tests and a database.The bacterium strain,which is isolated from the environment,can be identified by this system if its Gram and oxidase are negative.To apply this system to the experiment is very effective to the improvement of students' ability.
Keywords:API
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