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| Mutants of Escherichia coli heat-labile enterotoxin and cholera toxin as mucosal adjuvants | |
| 作者姓名： | 冯强 |
| 作者单位： | FENG Qiang 1，2，3，CAI Shaoxi 1，2，ZOU Quanming 4 1College of Bioengineering，Chongqing University，Chongqing 400044，P.R. China 2Key lab for Biomechanics and Tissue Engineering Under the State Minitery of Education，Chongqing University，Chongqing 400044，P.R. China 3Chongqing Education College，Chongqing 400067，P.R. China 4Department of Clinical Microbiology，Third Military Medical University，Chongqing 400038，P.R. China Received 9 October 2003; revised 1 December 2003 |
| 摘 要： | Heat-labile enterotoxin(LT) and cholera toxin(CT) are highly homologous (82 % at the amino acid level), they are the causative factors of travelers diarrhea and cholera respectively1]. When co-stimulating the surface of the mucosal with antigens, these toxins induce significantly immunological reaction 2]. Because of this character, LT and CT are widely used as mucosal adjuvants in non-human animal models. But the high toxicity and allergenicity caused by IgE antibody (Ab) responded to b… |
Mutants of Escherichia coli heat-labile enterotoxin and cholera toxin as mucosal adjuvants |
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| FENG Qiang,CAI Shaoxi,ZOU Quanming College of Bioengineering,Chongqing University,Chongqing,P.R. China Key lab for Biomechanics and Tissue Engineering Under the State Minitery of Education,Chongqing University,Chongqing,P.R. Ch.Mutants of Escherichia coli heat-labile enterotoxin and cholera toxin as mucosal adjuvants[J].Journal of Chongqing University,2003,2(2). | |
| Authors: | FENG Qiang CAI Shaoxi ZOU Quanming College of Bioengineering Chongqing University Chongqing PR China Key lab for Biomechanics and Tissue Engineering Under the State Minitery of Education Chongqing University Chongqing PR Ch |
| Institution: | 1. College of Bioengineering, Chongqing University, Chongqing 400044, PR.China;Key lab for Biomechanics and Tissue Engineering Under the State Minitery of Education,Chongqing University, Chongqing 400044, P.R.China;Chongqing Education College, Chongqing 4000 2. College of Bioengineering, Chongqing University, Chongqing 400044, PR.China;Key lab for Biomechanics and Tissue Engineering Under the State Minitery of Education,Chongqing University, Chongqing 400044, P.R.China 3. Department of Clinical Microbiology, Third Military Medical University, Chongqing 400038, P.R.China |
| Abstract: | Mucosal vaccination has been getting more and more recognition because of its compliance and low risk of spreading infectious disease by contaminated syringes used in subcutaneous immunization. However, most vaccines are unable to induce immune responses when given mucosally, and require the use of strong adjuvant for effective delivery systems. Heat-labile enterotoxin (LT) and Cholera toxin(CT) are powerful mucosal adjuvants when co-administered with soluble antigens. But high toxicity hampers their use in humans. Thanks to the fine knowledge of the structure-function relationship of LT and CT, many nontoxic or low toxic mutants have been generated, part of them retain high adjuvanticity of mucosal immunization. Among these mutants, LTS63K, LTA72R, LTR192G and CTE29H, CTE112K have been widely investigated. LTS63K and CTE112K are fully non toxic, whereas LTA72R and CTE29H are low toxic, and LTR192G is nontoxic in vitro(it remains the same toxicity as wild type LT in vivo). These mutants are extremely active as mucosal adjuvants when co-administrated with a variety of antigens in different animal models. They will be investigated more widely and deeply in the future. Some of them will be tested soon in human bodies. |
| Keywords: | mutants mucosal adjuvant heat-labile enterotoxin cholera toxin |
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