The nucleotide sequence of Shigella flexneri 1A: A common Indian isolate |
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| Authors: | Suvidya H. Ranade Ashraf Hossani D. N. Deobagkar D. D. Deobagkar B. A. Chopade Pramod S. Khandekar |
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| Affiliation: | (1) Department of Chemistry, University of Pune, Pune, India;(2) Department of Zoology, University of Pune, Pune, India;(3) Department of Microbiology, University of Pune, Pune, India;(4) Department of IBB, University of Pune, Pune, India;(5) Sinhagadh College of Engineering, Pune, India;(6) Department of Chemistry, University of Pune, Pune, 411007, Maharashtra, India |
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| Abstract: | We report complete sequences of 2 genes of S.flexneri 1a an Indian isolate for the first time. Shigella is causative agent of shigellosis or bacillary dysentery. Genomic library was constructed by shotgun approach. Sequencing was carried out using Big Dye terminator chemistry using ABI 3730 48 capillary DNA analyzer. 170 recombinants were subjected to nucleotide sequencing. Sequence data was analyzed, of these 2 clones showed presence of complete genes out of the total clones sequenced. Annotations were done using various bioinformatics tools. Gene Sfo676 on contig SF21B11, 513 bp long codes for a protein 170 aa long with molecular weight of 18836.5 daltons. The protein is 99 % identical to S. flexneri 2a 301 and not with any other strain of Shigella. It has 7 different sites for phosphorylation, myristoylation and glycosylation. Predicted cellular localization is cytoplasmic membrane. SF0368 is another full-length gene SF0368 on contig SF69C1 is a 312 nucleotide long. It is 103 aa long with molecular weight 11394.0 daltons. Protein is 100% identical to S. flexneri 2a 301 and 99% with S. sonnei strain 046. The gene shows difference when compared with S.sonnei in mono and dinucleotide frequency as well as amino acid composition. |
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| Keywords: | Shigella Flexneri 1a Shotgun sequencing Cytoplasmic membrane Shibase |
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