| 吴茱萸碱诱导人乳腺癌MCF-7细胞凋亡 |
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| 引用本文： | 于海帅,刘春禹. 吴茱萸碱诱导人乳腺癌MCF-7细胞凋亡[J]. 技术与教育, 2008, 0(1) |
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| 作者姓名： | 于海帅 刘春禹 |
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| 作者单位： | 吉林工业职业技术学院化学工程系;吉林省中医院针灸科; |
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| 摘 要： | 通过用MTT法测定药物对MCF-7细胞的细胞毒作用,倒置显微镜观察细胞形态,LDH法测定凋亡和坏死的比率,Western blot方法分析药物作用后对MCF-7细胞中Bcl-2家族蛋白的表达,得出了在MCF-7细胞凋亡过程中,吴茱萸碱上调Bcl-2蛋白表达,下调Bax表达的结论。
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| 关 键 词： | 吴茱萸碱 人乳腺癌MCF-7细胞 凋亡 Bcl-2家族蛋白 |
Apoptosis of Galactophore Cancer Cell of Human Induced by Evodiamine |
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| YU Hai-shuai LIU Chun-yu. Apoptosis of Galactophore Cancer Cell of Human Induced by Evodiamine[J]. Technique&Education, 2008, 0(1) |
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| Authors: | YU Hai-shuai LIU Chun-yu |
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| Affiliation: | YU Hai-shuai1 LIU Chun-yu2 (1 Chemical Engineering Dept,Jilin Vocational College of Industry , Technology,Jilin 132013,China)(2 Acupuncture Dept,Herbalist Hospital of Jilin,Changchun 130021,China) |
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| Abstract: | The cytotoxicity of drug to MCF-7 cell is mensurated by MIT,and the conformation of cell is observed by a microscope in inversion. The ratio of apoptosis and necrosis of cell is mensurated by LDH;the expression of Bcl-2 family of MCF-7 cell after the drug acted is analyzed by western blot method.And a conclusion is received that evodiamine up-regulatees the expression of Bcl-2 and down-regulates the expression of Bax in the apoptosis of cell. |
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| Keywords: | evodiamine galactophore cancer cell apoptosis Bcl-2 family protein |
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