Spontaneous alternation behavior in Paramecium

Spontaneous alternating behavior (SAB), the tendency of an organism to successively turn left and right in a maze, is a standard metric of short-term memory in developmental and pyschopharmacological studies. Although SAB is perhaps the most phylogenetically widespread behavior in motile lifeforms, conflicting data on SAB exist for several sets of closely related taxa. For example, previous studies provide contradictory evidence concerning the existence of spontaneous alternation behavior in the protozoanParamecium. However, these studies tested different species, using very different experimental designs. Using a single, factorial design for both previously tested species,P. multimicronucleatum andP. caudatum, we found that, in fact, the two species do not differ in their alternation behavior; specifically, they alternate in mazes with short tracks, but not in mazes with long tracks. These results are in accord with alternation studies on other taxa, and they fully resolve the apparent contradictions in the earlier studies onParamecium. They also indicate that caution should be used in interpreting variable results across species tested with different experimental designs and suggest that, in at least some taxa, SAB may not be strictly dependent on the use of short-term memory.