| 浊度/分光光度法与毛细管等电点聚焦法测定明胶等电点及其分布的比较研究（英文） |
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| 引用本文： | 陆扬,彭必先.浊度/分光光度法与毛细管等电点聚焦法测定明胶等电点及其分布的比较研究（英文）[J].中国科学院研究生院学报,2007,24(1):114-118. |
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| 作者姓名： | 陆扬 彭必先 |
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| 作者单位： | 1. 中国科学院理化技术研究所,北京,100080;中国科学院研究生院,北京,100049
2. 中国科学院理化技术研究所,北京,100080 |
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| 摘 要： | 等电点是蛋白质最重要的物理化学性质之一。目前有多种测定等电点的方法。本文利用最简单的浊度/分光光度法和最先进的毛细管等电点聚焦法分别测定了河南牛皮明胶的等电点，对两种方法进行比较并论证了后一种方法的优势。
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| 关 键 词： | 等电点 明胶 浊度/分光光度法 毛细管等电点聚集法 |
| 文章编号： | 1002-1175(2007)01-0114-05 |
| 修稿时间： | 2006年1月20日 |
Comparative Study of Determining IEP and IEP Distribution of Gelatin with Turbidimetry/Spectrometry and Capillary IEP Focusing Methods |
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| LU Yang,PENG Bi-Xian.Comparative Study of Determining IEP and IEP Distribution of Gelatin with Turbidimetry/Spectrometry and Capillary IEP Focusing Methods[J].Journal of the Graduate School of the Chinese Academy of Sciences,2007,24(1):114-118. |
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| Authors: | LU Yang PENG Bi-Xian |
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| Institution: | Technical Institute of Physics and Chemistry, Chinese Academy of Science
Graduate University of the Chinese Academy of Science |
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| Abstract: | The isoelectric point(IEP) is one of the most important physicochemical properties of proteins. There are many different methods for the determination of IEP. In this paper,a comparative study between the simplest turbidimetry/spectrometry method and the most advanced capillary IEP focusing(CaIPF) method was made. Henan cattle hide gelatin's IEP was determined with the two methods and the advantages of the CaIPF method over the other methods were demonstrated and discussed. |
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| Keywords: | isoelectric point gelatin turbidimetry/spectrometry capillary IEP focusing |
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