| 生物芯片微反应腔系统设计综述 |
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| 引用本文： | 范茂彦,张丽芳,沐仕光.生物芯片微反应腔系统设计综述[J].玉溪师范学院学报,2008,24(8):42-47. |
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| 作者姓名： | 范茂彦 张丽芳 沐仕光 |
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| 作者单位： | 玉溪师范学院,云南,玉溪,653100 |
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| 摘 要： | 聚合酶链式反应(PCR)在生命科学研究及诸多相关领域已经得到了广泛应用.充分利用等离子体干法刻蚀和各向异性湿法腐蚀的优点,先干法刻蚀,然后进行湿法腐蚀,可以制作出图形准确、底部致密平整、表面光滑、线条较直的PCR生物芯片微反应腔.而通过分析优化加热器材料和温度传感器的形状及尺寸,则可以满足DNA扩增反应对温度均匀性的要求.总体来看,PCR-DNA微阵列杂交集成的生物芯片在某种程度上是信息生物芯片(informalion-bio-chip)和功能生物芯片(function-biochip)的有机集成,但还不够完善,真正意义上的生物微系统应是功能生物芯片与信息生物芯片的进一步组合的产物.
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| 关 键 词： | 微反应腔 湿法腐蚀 等离子刻蚀 温度控制 微流控系统 |
Review of Biochip Reaction Chamber System Design |
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| FANG Mao-yan,ZHANG Li-fang,MU Shi-guang.Review of Biochip Reaction Chamber System Design[J].Journal of Yuxi Teachers' College,2008,24(8):42-47. |
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| Authors: | FANG Mao-yan ZHANG Li-fang MU Shi-guang |
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| Institution: | FANG Mao-yan ZHANG Li-fang MU Shi-guang(Yuxi Teachers College,Yuxi,Yunnan 653100) |
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| Abstract: | The polymerase chain reaction(PCR) has been extensively applied in the life science research and other fields.The PCR biochip reaction chamber with accurate shape,compact bottom,smooth surface and straight line can be fabricated by the combination of the first plasma dry etching and subsequent anisotropic wet etching.The requirement of DNA amplified reaction for temperature uniformity can be satisfied by the analysis of the shape and size of the heater material and temperature sensor.As a whole,the biochip ... |
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| Keywords: | biochip reaction micro-chamber Wet etching Plasma etching |
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