霍山石斛多糖干预对力竭运动小鼠BUN、MDA、BLA、肝糖原影响的研究

研究目的：通过建立小鼠负重游泳模型,从负重游泳力竭时间、血乳酸、血清尿素氮、丙二醛、肝糖元等指标实验研究,探讨霍山石斛多糖抗疲劳作用机制,为运动饮料中增加霍山石斛多糖提高运动员抗疲劳能力提供依据。研究方法：将SD小鼠随机分为4组。阴性对照组：给予生理盐水灌胃;阳性对照组：给予葡萄糖2g/（kg.d）灌胃;高剂量实验组：给予霍山石斛多糖450 mg/（kg.d）灌胃;低剂量实验组：给予霍山石斛多糖150mg/（kg.d）灌胃,实验组小鼠连续14d进行负重游泳力竭运动和给药后,测量和分析其血乳酸、血清尿素氮、丙二醛、肝糖元等指标变化。研究结果：1.霍山石斛多糖能显著延长小鼠游泳时间,降低运动后小鼠的乳酸累积。2.霍山石斛多糖能降低血清尿素氮、丙二醛含量,增加运动后小鼠的肝糖原储备值。3.补充霍山石斛多糖可以有效的提高小鼠的运动能力。

Effects of Intervention of Dendrobium Huoshanness Polysaccharide on BUN,MDA,BLA and Liver Glycogen in Mice after Exhaustive Exercise Protocol

Polysaccharide from Dendrobium huoshanense as anti-fatigue resistant composition studies has not been reported.From the angle of indicators changed as： the time of mouse to exhaust、BLA、BUN、MDA、Liver Glycogen,discussion on huoshanness polysaccharide anti-fatigue effect of intervention mechanisms provides a basis that sports drink which added Dendrobium huoshanense polysaccharides to improve the anti fatigue ability of athletes.METHODS： The SD mice were divided into four groups,each 8 mice（n=8） which are negative control group,given normal saline orally;positive control group,given Glucose 2g/（kg.d） orally;Polysaccharide huoshanness high-dose treatment group,given Polysaccharide huoshanness 450 mg/（kg.d） orally;Polysaccharide huoshanness low-dose treatment group,given Polysaccharide huoshanness 150 mg/（kg.d） orally,Continuous administration of 14d.From the angle of indicators changed as： the time of mouse to exhaust,BLA,BUN,MDA,Liver Glycogen,it discussed huoshanness polysaccharide anti-fatigue effect of intervention mechanisms.CONCLUSIONS： 1.The supplemented Dendrobium huoshanense polysaccharides can significantly increased the time of mouse to exhaust,reduce the accumulation of lactic acid in mice.2.The supplemented Dendrobium huoshanense polysaccharides can reduce the BUN,MDA and significantly increased the liver Glycogen of mice.3.The supplemented Dendrobium huoshanense polysaccharides can effectively improved the exercise capacity.