| 茶树EST资源中SSR的信息分析 |
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| 引用本文： | 金基强,李素芳,龚晓春,卢美贞,姚艳玲,忻雅,崔海瑞.茶树EST资源中SSR的信息分析[J].科技通报,2006,22(4):471-476. |
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| 作者姓名： | 金基强 李素芳 龚晓春 卢美贞 姚艳玲 忻雅 崔海瑞 |
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| 作者单位： | 1. 浙江大学,原子核农业科学研究所,杭州,310029
2. 中国计量学院,生命科学学院,杭州,310018 |
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| 基金项目： | 浙江省杭州市农业科学院基金 |
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| 摘 要： | 从NCBI公共数据库下载获得1989条茶树EST,去除其中的低质量的和冗余的序列,得到全长为734．54 kb的1589条无冗余EST。共在这些序列中发掘出了281个EST-SSR,分布于246条EST中,出现频率是17．68%。这些EST-SSR的平均长度为33．06 bp,平均分布频率是1/2．61 kb。茶树EST—SSR中,二核苷酸重复是主要的重复类型,出现最多的重复基元类型是AG/CT重复。本研究为茶树EST-SSR标记的建立和进一步应用奠定了基础。
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| 关 键 词： | 茶树 频率 特性 |
| 文章编号： | 1001-7119（2006）04-0471-06 |
| 收稿时间： | 2005-06-24 |
| 修稿时间： | 2005年6月24日 |
Analysis of SSR Information in EST Resource of Tea Plants(Camellia sinensis) |
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| JIN Ji-qiang,LI Su-fang,GONG Xiao-chun,LU Mei-zhen,YAO Yan-ling,XIN Ya,CUI Hai-rui.Analysis of SSR Information in EST Resource of Tea Plants(Camellia sinensis)[J].Bulletin of Science and Technology,2006,22(4):471-476. |
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| Authors: | JIN Ji-qiang LI Su-fang GONG Xiao-chun LU Mei-zhen YAO Yan-ling XIN Ya CUI Hai-rui |
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| Institution: | 1.Institute of Nuclear and Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 2. College of Life Science, China Jiliang University, Hangzhou 310018, China |
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| Abstract: | 1989 ESTs of tea plants were downloaded from the public database of NCBI and some of them redundant or with low quality were removed,finally resulting in 1589 non-redundant ESTs with total length of 734.54 kb.Totally 281 SSRs distributed in 246 ESTs were mined out,accounting for 17.68% of the non-redundant ESTs.The average length of tea plants EST-SSRs searched is 33.06 bp and the average distance of distribution is 1/2.16 kb.The dinueleotide repeat is the dominant type with repeat motif AG/CT being the most common.The result in this paper provides a base for the development and future application of EST-SSR markers in tea plants. |
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| Keywords: | EST-SSR |
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