| 拟南芥和大蒜中超氧化物歧化酶提取方法的比较 |
| |
| 引用本文： | 吴嘉,张美丽,杨红玉.拟南芥和大蒜中超氧化物歧化酶提取方法的比较[J].昆明师范高等专科学校学报,2012(6):55-56,59. |
| |
| 作者姓名： | 吴嘉 张美丽 杨红玉 |
| |
| 作者单位： | [1]昆明医科大学基础医学院,云南昆明650500 [2]昆明学院生命科学与技术系,云南昆明650214 |
| |
| 基金项目： | 国家自然科学基金资助项目（31260062）;昆明学院大学生创新性实验资助项目（x1008） |
| |
| 摘 要： | 用热变性法或磷酸缓冲液法从拟南芥植物中粗提取超氧化物歧化酶（SOD），再用丙酮法或硫酸铵分级盐析法纯化SOD．比较发现，热变性法粗提取的SOD回收率较高，而2种纯化方法的差异却无统计学意义；同时，还比较了用同一种方法所提取的拟南芥和大蒜之间的SOD活性，大蒜的SOD活性高于拟南芥．
|
| 关 键 词： | 拟南芥 大蒜 超氧化物歧化酶 分离纯化 |
Comparison of Extraction Method in Arabidopsis Thaliana and Garlic Superoxide Dismutase |
| |
| WU Jia,ZHANG Mei-li,YANG Hong-yu.Comparison of Extraction Method in Arabidopsis Thaliana and Garlic Superoxide Dismutase[J].Journal of Kunming Teachers College,2012(6):55-56,59. |
| |
| Authors: | WU Jia ZHANG Mei-li YANG Hong-yu |
| |
| Institution: | 1. Elementary Medical College, Kunming Medical University, Yunnan Kunming 650500, China; 2. Department of Life Sciences and Technology, Kunming University, Yunnan Kunming 650214, China) |
| |
| Abstract: | Thermal denaturation and phosphate buffer methods were applied to initial extraction of superoxide dismutase （SOD） from Arabidopsis thaliana plants, garnish with acetone method and ammonium sulfate classification salting out method were conducted to purify SOD. In result, the thermal denaturation method showed higher SOD recovery rate in extracting SOD comparing to the phosphate buffer method. SOD extracted using the two methods showed no difference in purification process. The conducted experiment concludes a SOD separation method of higher activity and higher purity. |
| |
| Keywords: | Arabidosis thaliana garlic SOD separation and purification |
| 本文献已被 维普 等数据库收录！ |
