中药多糖对耐力训练大鼠外周血T淋巴细胞亚群及活化T细胞数量的影响

目的探讨2种中药多糖对耐力训练大鼠外周血T淋巴细胞亚群和活化T细胞数量的作用,为改善耐力训练引起的细胞免疫功能低下寻找有效的干预措施。方法120只雄性Wistar大鼠随机分为6组,即耐力训练+黄芪多糖组、耐力训练+牛膝多糖组、单纯耐力训练组、安静+黄芪多糖组、安静+牛膝多糖组、安静对照组。每个组中均分为4 w组(7只大鼠)和6 w组(13只大鼠)。大鼠进行6 w递增负荷游泳训练后,采用流式细胞术观察大鼠外周血T淋巴细胞亚群及活化T细胞数量。结果①6 w耐力训练后可造成大鼠T细胞亚群中CD4+细胞数量及CD4+/CD8+比值下降,黄芪多糖和牛膝多糖有防止训练大鼠上述免疫指标下降的作用。②6 w耐力训练后可造成大鼠外周血活化T细胞数量的下降,黄芪多糖和牛膝多糖有防止训练大鼠外周血活化T细胞数量下降的作用。结论2种中药多糖有预防耐力训练大鼠外周血CD4+细胞数量及CD4+/CD8+比值和活化T淋巴细胞数量下降的作用。

Effect of Supplementation of Herbal Polysaccharide Extracts on Quantities of Peripheral T Lymphocyte Subsets and Activated T Lymphocytes in Rats Performing Endurance Training

Objective: In order to search potent intervening means for low-level exercise-associated cellular immune capacities,effect of supplementation of herbal polysaccharide extracts on quantities of peripheral T lymphocytes subsets and T lymphocytes in exercising rats was observed.Methods: 120 Wistar Rats were randomly divided into 6 groups: sedentary control group(n=20),sedentary with supplementation of APS group(n=20),sedentary with supplementation of ABPS group(n=20),training group(n=20),training with supplementation of APS group(n=20) and training with supplementation of ABPS group(n=20).Exercising rats took an incremental load protocol of swimming.After 4-week and 6-week training,numbers of peripheral T lymphocytes subsets and T lymphocytes in circulating blood were tested by FCM.Results: After 6-week training,(1)In training group,numbers of CD4~ and the ratio of CD4~ to CD8~ in T lymphocyte subsets,as well as were lower than that in sedentary control group(P<0.05).(2) Amounts of circulating activated T lymphocytes were declined in trained rats.(3) Blood indexes above mentioned were not decreased in rats supplemented with ABPS or APS(P>0.05).Conclusion: The results indicated that supplementation with ABPS or APS may help prevent the ratio of CD4~ to CD8~ and numbers of CD4~ as well as activated T lymphocyte in circulating blood from reducing.