| 蘑菇组织膜—二茂铁修饰的L-酪氨酸传感器 |
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| 引用本文： | 马全红,邓家祺. 蘑菇组织膜—二茂铁修饰的L-酪氨酸传感器[J]. 东南大学学报, 2000, 16(1): 106-110 |
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| 作者姓名： | 马全红 邓家祺 |
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| 作者单位： | 东南大学化学化工系,南京,210096,复旦大学化学系,上海,200433 |
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| 摘 要： | 本文采用二茂铁作为电子传递介体,利用蘑菇组织含有丰富的酪氨酸酶,将蘑菇组织肉浆固定在二茂铁(PVC膜)修饰石墨电极上,用电流法测定L-酪氨酸浓度.研究了电极的制备和最佳实验条件,其线性响应范围为2.0×10-4～4.5×10-3 mol.L-1,响应时间小于5 min,电极寿命至少30?d,可用于实际样品分析.，A new approach for assembling amperometric mushroom pulp tissue-based membrane electrode for determination of L-tyrosine analysis is proposed. Ferrocene is used as a mediator of electron transfer between tyrosinase in mushroom tissue and a graphite electrode. The optimal operation conditions are studied. The linear response range of the biosensor is 2.0×10-4to 4.5×10-3mol.L-1 with response time of less than 5 min and lifetime of at least 30 d. The biosensor can be applied to practical sample analysis.
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| 关 键 词： | 生物传感器 组织电极 修饰电极 二茂铁 L-酪氨酸 biosensor tissue-based membrane electrode，生物传感器 组织电极 修饰电极 二茂铁 L-酪氨酸 biosensor tissue-based membrane electrode |
Mushroom Pulp Tissue-Based Membrane-Ferrocene-Modified L-Tyrosine Biosensor |
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| Abstract: | |
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| Keywords: | biosensor tissue based membrane electrode modified electrode ferrocene L tyrosine |
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