日本血吸虫(大陆株)成虫低密度脂蛋白受体初探

利用非离子去污剂ＴｒｉｔｏｎＸ－１００抽提日本血吸虫成虫表膜蛋白，反相高效液相色谱（ＨＰＬＣ）分离纯化后，收集各主要蛋白峰，以１２５Ｉ标记的人血浆低密度脂蛋白（ＬＤＬ）作为放射性配基，通过放射性自显影初步鉴定表明日本血吸虫成虫表膜具有可与人血浆ＬＤＬ结合的受体蛋白。结果显示，低密度脂蛋白受体保留时间约为１１ｍｉｎ时，经ＳＤＳ－ＰＡＧＥ电泳鉴定其分子量约６００００，此蛋白能特异结合碘标记人血浆ＬＤＬ。本实验为了解血吸虫在宿主体内获得脂质的途径及进一步研制新的抗血吸虫药物打下基础。

Study on a Possible Low Density Lipoprotein Receptor of Adult Schistosoma japonicum in China Mainland

With Triton X 100,we extracted membrane protein of adult worms of Schistosoma japonicum.The putative lipoprotein receptor was purified through reverse phase high performance liquid chromatography (HPLC).The receptor was identified through autoradiographe using Iodine 125 labeled LDL as ligand.The result indicated the peak with the retention time of approximately 11 minutes bound ligand and the molecular weight was about 60 000.The research might open the way to obtain a new medicine against schistosoma.