| RP-HPLC-DAD法测定知母药材中菝葜皂苷元的含量 |
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| 引用本文： | 王良贵.RP-HPLC-DAD法测定知母药材中菝葜皂苷元的含量[J].丽水学院学报,2009,31(2):37-39. |
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| 作者姓名： | 王良贵 |
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| 作者单位： | 丽水学院,化学与生命科学学院,浙江,丽水,323000 |
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| 摘 要： | 应用RP-HPLC-DAD和差减光谱法检测知母药材中菝葜皂苷元的含量,探讨监控知母药材质量的可靠手段。结果表明,在甲醇流速0.7mL/min、柱温26℃、以DAD为检测器时得到较好的色谱分离效率,工作线性范围0.02～0.12 mg/mL,r=0.998 2,对药材的回收率为96.83%。
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| 关 键 词： | RP-HPLC-DAD 知母 菝葜皂苷元 含量测定 |
A Study on Determination of Sarsasapogenine in Ahemarrhena asphodoLoides Bunge by RP-HPLC-DAD |
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| Wang Lianggui.A Study on Determination of Sarsasapogenine in Ahemarrhena asphodoLoides Bunge by RP-HPLC-DAD[J].Journal of Lishui University,2009,31(2):37-39. |
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| Authors: | Wang Lianggui |
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| Institution: | College of Chemistry and Life Science Lishui University;Lishui Zhejiang 323000;China |
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| Abstract: | A method for the determination of the content of sarsasapogenine in Ahemarrhena asphodo Loides Bunge （A.A. Bunge） was established by reversed-phase high performance liquid chromatography with a diode array detector （RP-HPLC-DAD） and spectrum subtraction. The experimental results indicate that the separation efficiency was preferable under the condition of mobile phase consisted of methyl alcohol flow 0.7mL/ min, column temperature at 26℃ and using diode array detector. The method linearity was within the range of 0.02-0.12 mg/mL （r=0.998 2）.The average recovery of the A.A.Bunge rhizoma was 96.83%. |
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| Keywords: | RP-HPLC-DAD |
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