| 几种植物叶片中硒含量的荧光分光光度法测定 |
| |
| 引用本文： | 赵军.几种植物叶片中硒含量的荧光分光光度法测定[J].毕节学院学报,2002,20(4):91-94. |
| |
| 作者姓名： | 赵军 |
| |
| 作者单位： | 毕节师专化学系,贵州,毕节,551700 |
| |
| 摘 要： | 植物叶片硒含量的测定是衡量环境硒含量的重要手段.用消化液处理叶片,将叶片中结合于硒化物中的硒转化成为游离的Se4+.然后用2,3-二氨基萘(DAN)酸性溶液络合,生成4,5-苯并苤硒脑(4,5-Benzopiaselenol)络合物.取该络合物用环己烷萃取后进行荧光分光光度测试,荧光强度与Se4+的浓度成正比,测出的荧光强度与标准相比较,计算出样品的硒的含量.该方法用于测定叶片中的硒含量,有较高灵敏度.
|
| 关 键 词： | 硒 荧光分光光度法 DAN |
Determination of Selenium Content in Foliage with Spectrophotofluorometry |
| |
| Zhao Jun.Determination of Selenium Content in Foliage with Spectrophotofluorometry[J].Journal of Bijie University,2002,20(4):91-94. |
| |
| Authors: | Zhao Jun |
| |
| Abstract: | Determination of selenium content in foliage is an important method to measure environmental selenium content.The selenium in foliage selenide was changed into mobile Se4+ after the foliage were treated by assimilation liquid.The Se4+ was complexed by DAN in acid solution into 4,5-Benzopiaselenol,which was measured with Spectro-photofluorometry. This method was used to determine selenium content and has higher sensitivity. |
| |
| Keywords: | Selenium Spectrophotofluorometry DAN |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
