| 银杏产黄酮内生菌的分离培养研究 |
| |
| 引用本文： | 汤晓,朱建华. 银杏产黄酮内生菌的分离培养研究[J]. 宁波职业技术学院学报, 2010, 14(2): 96-101 |
| |
| 作者姓名： | 汤晓 朱建华 |
| |
| 作者单位： | 宁波职业技术学院,应用化工系,浙江,宁波,315800 |
| |
| 摘 要： | 从银杏根与茎中分离筛选出9株产黄酮类物质的内生菌,分别属于匐柄霉、暗梗梭孢霉属、交链孢属、镰孢霉属、蜜孢霉和放线菌属。这些内生菌以真菌为主,并首次发现一株放线菌也产黄酮。内生菌产黄酮的能力以槲皮素衡量,槲皮素产量最高可达9.14 mg/L。内生菌体外培养的传代稳定性较差。HPLC测定结果显示,银杏内生菌均分可分泌一种非黄酮类物质的成分。
|
| 关 键 词： | 银杏 内生菌 黄酮类物质 槲皮素 传代 HPLC |
Research for isolation and cultivation of flavone-producing endophytes from Ginkgo biloba.L |
| |
| TANG Xiao,ZHU Jian-hua. Research for isolation and cultivation of flavone-producing endophytes from Ginkgo biloba.L[J]. Journal of Ningbo Polytechnic, 2010, 14(2): 96-101 |
| |
| Authors: | TANG Xiao ZHU Jian-hua |
| |
| Affiliation: | Ningbo Polytecnic/a>;Department of applied chemical engineering/a>;Ningbo 315800/a>;China |
| |
| Abstract: | Nine flavone-producing endophytes were isolated from the root and the stem of Ginkgo biloba.L,which respectively belonged to Stemphylium sp,Fusella Sacc,Alternaria sp,Fusarium sp,Sphacelia sp and Actinomycea.These endophytes were mainly fungi,and a strain of actinomycete was found too.The flavoneproducing ability was measured by the production of quercetin,which a maximum could reach to 9.14mg/L.However,the vitro cultivation of these endophytes were not stable in generation passage.The HPLC results showed t... |
| |
| Keywords: | HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
