Optimization of elastolysis conditions and elastolytic kinetic analysis with elastase from Bacillus licheniformis ZJUEL31410 |
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Authors: | Qi-he Chen Guo-qing He |
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Institution: | (1) Department of Food Science and Nutrition, Zhejiang University, Hangzhou, 310029, China |
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Abstract: | The solubilization of elastin by Bacillus licheniformis elastase cannot be analyzed by conventional kinetic methods because the biologically relevant substrate is insoluble and
the concentration of enzyme-substrate complex has no physical meaning. In this paper we report the optimization of elastolysis
conditions and analysis of elastolytic kinetics. Our results indicated that the hydrolyzing temperature and time are very
important factors affecting elastolysis rate. The optimized conditions using central composite design were as follows: elastolysis
temperature 50 °C, elastase concentration 1×104U/ml, elastin 80 mg, elastolytic time 4 h. Investigation of the effects of substrate content, elastase concentration and pH
was also revealed that low or high elastin content inhibits the elastolysis process. Increasing elastase improves elastin
degradation, but high elastase may change the kinetics characterization. Alkaline environment can decrease elastin degradation
rate and pH may affect elastolysis by changing elastase reaction pH. To further elucidate the elastolysis process, the logistic
model was used to elastolysis kinetics study showing clearly that the logistic model can reasonably explain the elastolysis
process, especially under lower elastase concentration. However, there is still need for more investigations with the aid
of other methods, such as biochemical and molecular methods.
Project (No. Y304203) supported by the Natural Science Foundation of Zhejiang Province, China |
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Keywords: | Bacillus licheniformis Elastase Elastolysis condition optimization Elastolytic kinetics |
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