Bubble-templated synthesis of nanocatalyst Co/C as NADH oxidase mimic |
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| Authors: | Jinxing Chen Xiliang Zheng Jiaxin Zhang Qian Ma Zhiwei Zhao Liang Huang Weiwei Wu Ying Wang Jin Wang Shaojun Dong |
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| Institution: | State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, China;State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;Department of Chemistry and Physics, Stony Brook University, Stony Brook, NY 11794, USA |
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| Abstract: | Designing highly active nanozymes for various enzymatic reactions remains a challenge in practical applications and fundamental research. In this work, by studying the catalytic functions of natural NADH oxidase (NOX), we devised and synthesized a porous carbon-supported cobalt catalyst (Co/C) to mimic NOX. The Co/C can catalyze dehydrogenation of NADH and transfers electrons to O2 to produce H2O2. Density functional theory calculations reveal that the Co/C can catalyze O2 reduction to H2O2 or H2O considerably. The Co/C can also mediate electron transfer from NADH to heme protein cytochrome c, thereby exhibiting cytochrome c reductase-like activity. The Co/C nanoparticles can deplete NADH in cancer cells, induce increase of the reactive oxygen species, lead to impairment of oxidative phosphorylation and decrease in mitochondrial membrane potential, and cause ATP production to be damaged. This ‘domino effect’ facilitates the cell to approach apoptosis. |
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| Keywords: | NADH oxidase H2O2 production oxidative phosphorylation |
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