四种有机溶剂对小鼠骨髓来源细胞体外生长和活力影响的量效关系分析

目的：观察乙醇、1.2-丙二醇、二甲基亚砜、吐温80对小鼠骨髓基质细胞（BMSC）、粒-巨噬系祖细胞（CFU-GM）和内皮细胞系细胞（BMEC）体外生长和活力的影响。并分析量效关系。方法：分别设置不同终体积分数有机溶剂的细胞培养体系及其对照。检测成纤维形成单位（CFU-F）和CFU-GM的集落生成率，用MTT比色法和台盼蓝排斥试验测定BMSC和BMEC的细胞活力和活细胞率。结果：乙醇为1.6%、丙二醇为1.6%、二甲基亚砜为2％、吐温80为0．005％-0．01％时，CFU-F和CFU-GM集落产率与对照纽相比显著降低（P〈0．05或〈0．01）。乙醇和丙二醇为02％、0．4％、二甲基亚砜为0.5％-1.0%、吐温80为0．002％-0．005％时，BMSC和CFU-GM的细胞活力和活细胞率与对照组相比显著减低（P〈0．05或〈0．01）。随着这几种有机溶剂在上述体积分数上增加，各相应检测指标的减低率递增。结论：乙醇、丙二醇、二甲基亚砜，吐温80在细胞培养体系中达一定含量时，对小鼠骨髓BMSC、CFU-GM和BMEC细胞具有明显的生长抑制作用和（或）细胞毒性效应。含量增高则效应更显著。

A Dose-Effect Analysis of Four Organic Solvents Influencing the Growth and Viability of Murine Bone Marrow-Derived Cells in Vitro

Objective: This study was purported to examine the effect of ethanol, propylene glycol (PEG), dimethyl sulfoxide (DMSO) and Tween 80 on the growth and viability of bone marrow stromal cell (BMSC), colony-forming units for granulocytes and macrophages (CFU-GM) and bone marrow endothelial cell line (BMEC) from murine bone marrow in vitro, and to analyze the concentration-effect relationship. Methods: The colony yields of colony-forming units fibroblastic (CFU-F) and CFU-GM were assessed in the murine bone mar...