分子印迹技术及应用

分子印记技术是在近十几年来才发展起来的一门边缘科学技术，它结合了高分子化学、生物化学等学科，作为一种新型高效分离技术，具有空间专一识别性，本文综述了共价法与非共价法分子印迹技术的基本原理，采用传统方法、扩散聚合、悬浮聚合和表面印迹等制备分子印迹聚合物的方法，以及分子印迹技术在色谱分离、抗体和受体模拟物、固相萃取、生物传感器等领域的应用，最后指出了分子印迹技术目前存在的问题。

Molecular Imprinting Technique and Its Applications

Molecular imprinting technique (MIT) is a borderline subject developed in recent several decades. It developed from combining high polymer chemistry and biochemistry. It is a new effective separation technique featuring selective recognition. Fundamental principle of the covalent and non-covalent approach is described. Different methods for preparing molecular imprinting polymer including conventional method, dispersion polymerization, suspension polymerization and surface imprinting are reviewed. Molecular imprinting polymers have applied in many fields which include the use of it as tailor-made separation materials, antibody and receptor binding site mimics in recognition and assay systems, solid phase extraction and as recognition elements in biosensors. Problems existing in molecular imprinting technique are pointed out also.