优化米曲霉固体发酵生产辅酶Q10的研究

以辅酶Q10产量为指标,对米曲霉2077固体发酵法生产辅酶Q10进行了初步系统性研究.利用配方均匀设计和正交设计方法分别对培养基和培养条件进行优化,建立了相应的数学模型,得到了较优固体培养基为大豆8.20%、麦麸63.53%和谷糠28.27%,确定100 g培养基固体培养条件为：料水比为1、青霉素钠加入量为3mL、发酵温度40℃、发酵时间96 h为较优工艺条件.在此优化培养基和培养条件下得到米曲霉2077产辅酶Q10的最大值为0.947/mg·g-1干培养基.

Study on production of coenzyme QIO with AspergiUus oryzae solid fermentation

Based on the yield of coenzyme Q10 as indicatior, the production of coenzyme Q10 with solid state fermentation of Aspergillus oryzae 2077 was studied in the paper. The culture medium and conditions were optimized separately by the uniform design of the formulation and the cultivation of the orthogonal design method, and the corresponding mathematical model was established. According to the analysis, the superior solid medium was set at soybean 8.20%, wheat bran 63.53% and rice bran 28.27%, and the optimum conditions of solid fermentation were as following： the atio of material and water was 1, the penicillin sodium addition 3 mL, fermentation temperature 40℃, fermentation time 96 h. On the optimization conditions, the maximum production of coenzyme Q10 was 0. 947/mg · g-1 by using Aspergillus oryzae solid fermentation.