胡枝子属部分植物的ITS序列分析

利用ITS序列分析技术对胡枝子属植物进行了亲缘关系分析,探讨其遗传多样性及其亲缘关系,试图为胡枝子属植物资源开发利用提供依据。将从Gen Bank检索获得的24条胡枝子属植物(19个种)的ITS序列做系统发育分析,Clustal 2.0软件进行序列比对,Mega4.0计算序列核苷酸比例及遗传距离,并构建邻接树(Neighbor-joining tree,NJ Tree)。胡枝子的19种植物ITS序列全长为622 bp,变异位点占总序列的16.6%;K-2-P遗传距离为0.001-0.085;鉴定成功率为57.9%,19种胡枝子可以聚为3支,与形态学分类结果并不完全一致。

Analysis of ITS Sequences in Lespedeza

Phylogenetic analysis of ITS in Lespedeza can explore the genetic diversity and phylogenetic relationship of this genus and provide the basis for plant resource exploitation in Lespedeza.24 ITS sequences（belong to 19 species） from Gen Bank Accession are obtained to analysize phylogenetic relationship, Clustal 2.0 and Mega4.0 softwares were used to caculate genetic distance and build the adjacent tree（Neighbor-joining tree, NJ Tree）.The length of ITS sequences was 622 bp, variation sites was16.6% of the total sequence; K-2-P genetic distance was 0.001-0.085; identification success rate was57.9%, 19 species can be clustered into three branch, which was not entirely consistent with the morphological classification.