Label-free isolation of a prostate cancer cell among blood cells and the single-cell measurement of drug accumulation using an integrated microfluidic chip |
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| Authors: | A Khamenehfar T V Beischlag P J Russell M T P Ling C Nelson P C H Li |
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| Institution: | 1.Department of Chemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada;2.Faculty of Health Sciences, Simon Fraser University, Burnaby, British Columbia V5A1S6, Canada;3.Australian Prostate Cancer Research Centre—Queensland, Institute of Health and Biomedical Innovation, Department Faculty of Health, Queensland University of Technology, Princess Alexandra Hospital, Translational Research Institute, Brisbane, Australia |
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| Abstract: | Circulating tumor cells (CTCs) are found in the blood of patients with cancer. Although these cells are rare, they can provide useful information for chemotherapy. However, isolation of these rare cells from blood is technically challenging because they are small in numbers. An integrated microfluidic chip, dubbed CTC chip, was designed and fabricated for conducting tumor cell isolation. As CTCs usually show multidrug resistance (MDR), the effect of MDR inhibitors on chemotherapeutic drug accumulation in the isolated single tumor cell is measured. As a model of CTC isolation, human prostate cancer cells were mixed with mouse blood cells and the label-free isolation of the tumor cells was conducted based on cell size difference. The major advantages of the CTC chip are the ability for fast cell isolation, followed by multiple rounds of single-cell measurements, suggesting a potential assay for detecting the drug responses based on the liquid biopsy of cancer patients. |
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