| 采用RT-PCR技术检测甜樱桃“红灯”上的PNRSV |
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| 引用本文： | 侯义龙,张立娟,于亚军,于大永. 采用RT-PCR技术检测甜樱桃“红灯”上的PNRSV[J]. 大连大学学报, 2006, 27(4): 59-60 |
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| 作者姓名： | 侯义龙 张立娟 于亚军 于大永 |
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| 作者单位： | 大连大学,生物工程学院,辽宁,大连,116622 |
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| 基金项目： | 辽宁省博士启动基金项目(20021052)，辽宁省教育厅高等学校科研项目(2004D202) |
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| 摘 要： | [目的]调查大连市特色水果品种甜樱桃“红灯”的田间植株携带PNRSV的情况．[方法]利用作者已建立的PNRSV RT-PCR检测体系．[结果]被检植株PNRSV的感染率达100％,但程度不同．[结论]对于其中感染PNRSV较轻的植株可以作为今后培育无PNRSV苗木的原材料．
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| 关 键 词： | 逆转录-聚合酶链式反应 PNRSV 检测 |
| 文章编号： | 1008-2395（2006）04-0059-02 |
| 收稿时间： | 2006-04-10 |
| 修稿时间： | 2006-04-10 |
Detection of PNRSV in sweetcherry varity HongDeng'''' by RT-PCR |
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| HOU Yi-long,ZHANG Li-juan,YU Ya-jun,YU Da-yong. Detection of PNRSV in sweetcherry varity HongDeng'''' by RT-PCR[J]. Journal of Dalian University, 2006, 27(4): 59-60 |
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| Authors: | HOU Yi-long ZHANG Li-juan YU Ya-jun YU Da-yong |
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| Abstract: | [Objective] To investigate whether the sweet cherry varity " gDeng" in hard was infected by PNRSV or not. [Methods] The RT-PCR detection system published for PNRSV was used. [Results] All the trees detected were infected by PNRSV,but the infected degree was different. [Conclusion] The trees which were not infected seriously will be chosen as the primary materials to culture the young plants with PNRSV-free. |
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| Keywords: | RT-PCR PNRSV Detection |
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