| A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana |
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| 引用本文： | CHEN Bing1,JIANG Jin-hua1,2,ZHOU Xue-ping1(1State Key Laboratory of Rice Biology,Institute of Biotechnology,Zhejiang University,Hangzhou 310029,China)(2College of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China). A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana[J]. Journal of Zhejiang University. Science. B, 2007, 0(4) |
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| 作者姓名： | CHEN Bing1 JIANG Jin-hua1 2 ZHOU Xue-ping1(1State Key Laboratory of Rice Biology Institute of Biotechnology Zhejiang University Hangzhou 310029 China)(2College of Life Sciences Nanjing Agricultural University Nanjing 210095 China) |
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| 作者单位： | CHEN Bing1，JIANG Jin-hua1，2，ZHOU Xue-ping1(1State Key Laboratory of Rice Biology，Institute of Biotechnology，Zhejiang University，Hangzhou 310029，China)(2College of Life Sciences，Nanjing Agricultural University，Nanjing 210095，China) |
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| 基金项目： | Project supported by the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China (No. 705025)，the National Natural Science Foundation of China (No. 30530520) |
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| 摘 要： | The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus(TMV) in A.thaliana.In this study,we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1.Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N.tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities,respectively.Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N.benthamiana.The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants.
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A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana |
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| CHEN Bing,JIANG Jin-hua,,ZHOU Xue-ping. A TOM1 homologue is required for multiplication of Tobacco mosaic virus in Nicotiana benthamiana[J]. Journal of Zhejiang University. Science. B, 2007, 0(4) |
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| Authors: | CHEN Bing JIANG Jin-hua ZHOU Xue-ping |
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| Affiliation: | CHEN Bing1,JIANG Jin-hua1,2,ZHOU Xue-ping1 |
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| Abstract: | The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus(TMV) in A.thaliana.In this study,we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1.Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N.tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities,respectively.Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N.benthamiana.The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants. |
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| Keywords: | Tobacco mosaic virus NbTOM1 Virus induced gene silencing Nicotiana benthamiana |
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