| 龙脷叶提取物体外抑菌活性初步研究 |
| |
| 引用本文： | 黄燕,谭建宁,马雯芳.龙脷叶提取物体外抑菌活性初步研究[J].大众科技,2014(2):68-70. |
| |
| 作者姓名： | 黄燕 谭建宁 马雯芳 |
| |
| 作者单位： | 广西中医药大学附属瑞康医院,广西 南宁530001 |
| |
| 基金项目： | 广西自然科学基金项目（2013GXNSFAA019171）广西中医药民族医药继承创新工程壮瑶医药研究与开发项目（GZZY13-10）. |
| |
| 摘 要： | 采用连续二倍梯度琼脂稀释法研究龙脷叶水提物和的50%、70%、95%乙醇提取物的抑菌活性,并对抑菌作用强的提取物以石油醚、乙酸乙酯、正丁醇依次萃取,测定不同极性部位的最低抑菌浓度(MIC)。结果表明,龙脷叶50%乙醇提取物的正丁醇部位对六种试验菌株明显抑制:对金黄色葡萄球菌(Staphylococcus aureus)、金黄色葡萄球菌耐药株(Methicillin resistant Staphylococcus aureus)和大肠杆菌(Escherichia coli)的MIC为15.60m g·mL-1;对铜绿假单胞菌(Pseudomonas aeruginosa)、伤寒沙门氏菌(Salmonella typhi.)和乙型副伤寒沙门氏菌(Bacillus paratyphosus B)的MIC为7.81m g·mL-1。可为进一步研究龙脷叶抑菌活性成分提供依据。
|
| 关 键 词： | 龙脷叶 提取物 最低抑菌浓度 |
Studies on antibacterial active of the extracts from Sauropus spatuli folius Beille in vitro |
| |
| Abstract: | The minimum inhibitory concentration （MIC） of system solvent extraction by petroleum, ethyl acetate and N-butanol from water and Ethanol Extract of Sauropus spatuli folius Beille against six strains were detected by using continuous double agar dilution method. The results indicated that The N-butanol liquor from Water Extract of Sauropus spatuli folius Beille had good antibacterial activities against the six strains .The MIC were 15.60m g＆#183;mL-1 on Staphylococcus aureus、Methicillin resistant Staphylococcus aureus and Escherichia coli. The MIC were 7.81 mg＆#183;mL-1 on Pseudomonas aeruginosa ,Salmonella typhi and Bacillus paratyphosus B. Sauropus spatuli folius Beille has antibacterial effects in vitro. Antibacterial active situs mainly existed in n-butanol. |
| |
| Keywords: | Sauropus spatuli folius Beille antibacterial MIC |
| 本文献已被 CNKI 维普 等数据库收录！ |
