| 产酶微生物筛选及其在帕罗西汀中间体拆分中的应用 |
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| 引用本文： | 何耀莹,骆晨涛.产酶微生物筛选及其在帕罗西汀中间体拆分中的应用[J].昆明师范高等专科学校学报,2011(3):95-97. |
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| 作者姓名： | 何耀莹 骆晨涛 |
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| 作者单位： | 浙江工业大学生物与环境工程学院; |
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| 摘 要： | 通过手性柱高效液相色谱（HPLC）分析法,从实验室保藏菌种以及采集的土样中筛选出能立体选择性催化帕罗西汀中间体的产脂肪酶的微生物菌株.其中,编号为油4的菌株所产的脂肪酶具有相对较高的催化活力和立体选择性,在反应温度为35℃,反应时间为14 h时,催化水解化反应的底物转化率为39.64%,产物对映体过量值为17.8%.
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| 关 键 词： | 帕罗西汀 立体选择性 高效液相色谱 对映体过量值 |
Screening of Enzyme-producing Microorganisms and their Application to the Chiral Resolution of Paroxetine Intermediate |
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| HE Yao-ying,LUO Chen-tao.Screening of Enzyme-producing Microorganisms and their Application to the Chiral Resolution of Paroxetine Intermediate[J].Journal of Kunming Teachers College,2011(3):95-97. |
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| Authors: | HE Yao-ying LUO Chen-tao |
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| Institution: | HE Yao-ying,LUO Chen-tao(College of Biological and Environmental Engineering,Zhejiang University of Technology,Zhjiang Hangzhou 310014,China) |
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| Abstract: | Using chiral HPLC as an analytical method,Lipase-producing strains able to enantioselectivily catalyze Paroxetine intermediate were isolated from laboratory strains and soil samples,among which You4 displayed the highest enantiomeric excess value.The reaction conversion rate was 39.64%,and e.e.value of substrate was 17.8% when at 35℃ in 14h.Strain You4 was identified as bacillus through a series of physiological tests. |
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| Keywords: | Paroxetine enantioselectivity chiral high performance liquid chromatography enantiomeric excess |
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