| 两种益生菌混合发酵乳清培养基的优化 |
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| 引用本文： | 范娜,陈雪峰.两种益生菌混合发酵乳清培养基的优化[J].商洛学院学报,2011,25(6):50-55. |
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| 作者姓名： | 范娜 陈雪峰 |
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| 作者单位： | 1. 商洛学院生物医药工程系,陕西商洛,726000
2. 陕西科技大学生命科学与工程学院,陕西西安,710021 |
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| 基金项目： | 陕西科技大学博士科研启动基金项目(BJ09-22) |
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| 摘 要： | 根据嗜酸乳杆菌和双歧杆菌的营养需要和生长特性,采用响应面分析法对其混合发酵乳清培养基进行优化研究。利用SAS中的Plackett-Burman二水平设计法成功选取了对嗜酸乳杆菌和双歧杆菌发酵培养影响显著的3个因素(大豆分离蛋白、西红柿汁和胡萝卜汁),再用最陡爬坡实验法确定因素水平,最后依据回归模型分析,得出最佳的混合发酵乳清培养基配方为:4%乳清粉、1.4%大豆分离蛋白、10%西红柿汁、13%胡萝卜汁、0.5%半胱氨酸盐酸盐、0.04%低聚异麦芽糖、0.3%K2HPO4。用此优化的培养基培养嗜酸乳杆菌和双歧杆菌,活菌数可高达9.8×109 cfu.mL-1。
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| 关 键 词： | 嗜酸乳杆菌 双歧杆菌 乳清培养基 |
Optimization of Two Kinds of Probiotics Mixed Fermentation on Whey Medium |
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| FAN Na,CHEN Xue-feng.Optimization of Two Kinds of Probiotics Mixed Fermentation on Whey Medium[J].Journal of Shangluo University,2011,25(6):50-55. |
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| Authors: | FAN Na CHEN Xue-feng |
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| Institution: | FAN Na1,CHEN Xue-feng2(1.Biopharmaceutical Engineering Department of Shangluo University,Shangluo,Shaanxi 726000,2.College of Life Science and Engineering,Shaanxi University of Science & Technology,Xi'an,Shaanxi 710021) |
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| Abstract: | The Lactobacillus acidophilus and Bifidobacterium mixed fermentation of whey medium was optimized by the response surface method according to the nutritional needs and growth characteristics of the strains.Three significant factors of Lactobacillus acidophilus and Bifidobacterium mixed fermentation cultivation were soybean protein isolated,tomato juice and carrot juice,which were selected via Plackett-Burman design of SAS soft.The factors level was determined by the steepest ascent experiment.Then the optim... |
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| Keywords: | Bifidobacterium Lactobacillus acidophilus whey medium |
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