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黑僵菌var.majus核型的电泳分析
引用本文:时连根,卢春松,王象礼,清水进.黑僵菌var.majus核型的电泳分析[J].科技通报,1998(5).
作者姓名:时连根  卢春松  王象礼  清水进
作者单位:浙江农业大学蚕学系(时连根),浙江德清蚕业公司(卢春松),浙江丝绸联合公司(王象礼),京都工艺纤维大学(清水进)
摘    要:用脉冲凝胶电泳(pulsedfieldgelelectrophoresis,PFGE)技术分析了黑僵菌var.majus(Metarhiziumanisopliaevar.majus)核型.得出黑僵菌var.majus至少有7条染色体,其大小在1.1~6.5Mbp之间,3菌株的核型大小为21.9~26.4Mbp.黑僵菌var.majus核型在菌株间存在多型性.

关 键 词:黑僵菌var.majus  核型  脉冲凝胶电泳

Karyotype Analysis of Metarhizium anisopliae var. majus by Pulsed field Gel Electrophoresis
Abstract:The karyotype of Metarhizium anisopliae var. majus was investigated by pulsed field gel electrophoresis (PFGE) technique. M.anisopliae var. majus had at least seven chromosomes,whose sizes were ranged from 1.1 to 6.5 megabase pairs (Mbp) and total genome sizes of the three isolates were estimated to be in the range from 21.9 to 26.4 Mbp.Significant differences in the size of each chromosome existed among the isolates of M.anisopliae var. majus .
Keywords:Metarhizium anisopliae  var    majus    Karyotype  Pulsed  field gel electrophoresis  
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