| 沼水蛙皮肤抗菌肽Temporin-HZ的结构和功能研究 |
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| 引用本文： | 黄颖桢,周建武,张洋,饶平凡. 沼水蛙皮肤抗菌肽Temporin-HZ的结构和功能研究[J]. 莆田学院学报, 2008, 15(5) |
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| 作者姓名： | 黄颖桢 周建武 张洋 饶平凡 |
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| 作者单位： | 1. 福州大学,生物工程研究所,福建,福州,350002;福建省农业科学院农业生物资源研究所,药用植物研究中心,福建,福州,350003
2. 福州大学,生物工程研究所,福建,福州,350002 |
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| 摘 要： | 应用反相高效液相色谱和质谱联合使用手段,从沼水蛙皮肤分泌液中分离到一种新型的Temporin家族成员——Temporin-HZ,经过Edman降解氨基酸序列分析和串联质谱研究,Temporin-HZ的一级结构最终确定为Phe-Leu-Pro-Leu-Leu-Phe-Gla-Ala-Ile-Ser-His-Leu-Leu-NH2。抗菌活性分析表明,Temporin-HZ对大肠杆菌和白色念珠菌生长无明显抑制,但对金黄色葡萄球菌有抗菌作用。
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| 关 键 词： | 沼水蛙 抗菌肽 Temporin-HZ 测序 最小抑菌浓度 |
Structural Characterization and Structional Analysis of Temporin-HZ from Skin Secretion of Hylarana Guentheri |
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| HUANG Ying-zhen,ZHOU Jian-wu,ZHANG Yang,RAO Ping-fan. Structural Characterization and Structional Analysis of Temporin-HZ from Skin Secretion of Hylarana Guentheri[J]. journal of putian university, 2008, 15(5) |
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| Authors: | HUANG Ying-zhen ZHOU Jian-wu ZHANG Yang RAO Ping-fan |
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| Affiliation: | HUANG Ying-zhen1,2,ZHOU Jian-wu1,ZHANG Yang1,RAO Ping-fan1 ( 1. Institute of Biotechnology,Fuzhou University,Fuzhou Fujian 350002,China,2. Medicinal Plant Research Center,Institute of Agricultural Bioresources,Fujian Acedamy of Agricultural Science,Fuzhou Fujian 350003,China ) |
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| Abstract: | A new temporin-HZ was isolated from the skin secretion of Hylarana guentheri by reverse phase HPLC and mass spectrometry analysis. The primary structure of temporin-HZ(Phe-Leu-Pro-Leu-Leu-Phe-Gla- Ala-Ile-Ser-His-Leu-Leu-NH2) was identified after Edman degradation amino acid sequencing and ms/ms Q-TOF analyzing. The temporin-HZ had moderate activity against Staphylococcus aureus and no activity against Escherichia coli and Candida albicans. |
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| Keywords: | Temporin-HZ |
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