| 毛细管电泳法测定苹果中维生素含量 |
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| 引用本文： | 曹慧,刘海兴,张玉宵.毛细管电泳法测定苹果中维生素含量[J].实验技术与管理,2011,28(4):31-32. |
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| 作者姓名： | 曹慧 刘海兴 张玉宵 |
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| 作者单位： | 潍坊学院,生物工程学院,山东,潍坊,261061 |
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| 基金项目： | 潍坊市科技发展计划项目资助，生命分析化学教育部重点实验室开放基金，潍坊学院优秀学术团队项目 |
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| 摘 要： | 采用毛细管区带电泳法测定了5种苹果中维生素C和烟酸的含量。在20mmol/L硼砂和20%乙腈的混合缓冲体系(pH 8.5)中,两种维生素在15min内较好地得到分离。维生素C和烟酸的迁移时间和峰面积的相对标准偏差分别为5.43%和2.35%、6.24%和2.49%。质量浓度在0.007 81～1 000mg/L范围内线性相关系数分别为0.9964、0.9945。该方法简单、准确,可作为果实品质测定的一种方法。
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| 关 键 词： | 苹果 维生素C 毛细管区带电泳 烟酸 |
Determination of vitamin in apples by capillary zone electrophoresis |
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| Cao Hui,Liu Haixing,Zhang Yuxiao.Determination of vitamin in apples by capillary zone electrophoresis[J].Experimental Technology and Management,2011,28(4):31-32. |
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| Authors: | Cao Hui Liu Haixing Zhang Yuxiao |
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| Institution: | (Bioengineer College,Weifang University,Weifang 261061,China) |
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| Abstract: | Vc and nicotinic acid of five kinds of apples were directly determined by capillary zone electrophoresis.Under the mixed electrolyte(pH8.5),which was made of 20mmol L-1 borate and 20% ethyl,both of them were well separated within 15 minutes.The RSDs of migration time were 5.43%,6.24%,and its peak areas were 2.35%,2.49%,respectively.Correlation coefficients were 0.9964 and 0.9945 within the concentration range from 0.0078125mg·mL-1 to 1.0mg·mL-1.The method was simple and accurate as a new method to determine fruit quality. |
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| Keywords: | apple vitamin C capillary zone electrophoresis nicotinic acid |
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