| Adsorption of the Enantiomers of Tryptophan on Stationary Phase Bonded with Bovine Serum Albumin |
| |
| 作者姓名： | 张凤宝 程明 李爽 刘亮 张国亮 |
| |
| 作者单位： | School of Chemical Engineering and Technology Tianjin University，School of Chemical Engineering and Technology Tianjin University，School of Chemical Engineering and Technology Tianjin University，School of Chemical Engineering and Technology Tianjin University，School of Chemical Engineering and Technology Tianjin University，Tianjin 300072 China Tianjin 300072 China Tianjin 300072 China Tianjin 300072 China Tianjin 300072 China |
| |
| 摘 要： | Chromatography has become the most generalmethod to carry out preparative enantiomeric separa-tions, especially for the production of pure pharma-ceuticals and pharmaceutical intermediates1,2]. En-antiomeric separation should be carried out on chiralstationary phases (CSPs) column due to its advan-tages over other techniques of enantiomeric separationavailable. However, CSPs often have a relatively lowadsorption capacity. In the past decades, severalkinds of CSPs have been developed3,4].…
|
| 关 键 词： | 传质 吸附等温线 色氨酸 对映体 牛血清蛋白 |
| 收稿时间： | 2005-12-02 |
Adsorption of the Enantiomers of Tryptophan on Stationary Phase Bonded with Bovine Serum Albumin |
| |
| ZHANG Fengbao,CHENG Ming,LI Shuang,LIU Liang,ZHANG Guoliang.Adsorption of the Enantiomers of Tryptophan on Stationary Phase Bonded with Bovine Serum Albumin[J].Transactions of Tianjin University,2006,12(5):359-363. |
| |
| Authors: | ZHANG Fengbao CHENG Ming LI Shuang LIU Liang ZHANG Guoliang |
| |
| Abstract: | Frontal analysis is frequently applied to measuring single or multi-component adsorption isotherms. In this work, the competitive adsorption isotherm data of two enantiomers of tryptophan were obtained by competitive frontal analysis. The stationary phase in the column was silica-immobilized bovine serum albumin (BSA) by the derivative method, and the mobile phase was a phosphate buffer. These isotherm data were fitted by the competitive Bilangmuir model. This model can account for the behavior of both tryptophan enantiomers and these profiles were found to fit the experimental band profiles (square error is 0.999 6). The parameters obtained were used in numerical calculations to predict the band profiles of the racemic mixtures of tryptophan. The equilibrium-dispersive model provides satisfactory prediction, with minor differences between the calculated and the experimental profiles. |
| |
| Keywords: | mass transfer adsorption isotherms enantiomeric separation equilibrium-dispersive model competitive Bilangmuir model tryptophan |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
