| 祁术的组织培养及快速繁殖技术研究 |
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| 引用本文： | 胡长玉,叶玉娟,郝祥霞,胡跃. 祁术的组织培养及快速繁殖技术研究[J]. 黄山学院学报, 2006, 8(5): 57-60 |
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| 作者姓名： | 胡长玉 叶玉娟 郝祥霞 胡跃 |
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| 作者单位： | 黄山学院,生命与环境科学学院,安徽,黄山,245041 |
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| 基金项目： | 安徽省教育厅自然科学基金 |
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| 摘 要： | 为研究祁术的组织培养及快速繁殖技术,选用祁术的叶片和下胚轴为外植体材料,接种于附加植物生长调节剂NAA,6-BA及其组合的MS固体培养基上.结果表明:下胚轴为外植体明显好于叶片,对于愈伤组织形成,叶片采用MS NAA(0.5mg/L) 6-BA(2mg/L);下胚轴MS NAA(0.5mg/L) 6-BA(2mg/L);MS NAA(0.5mg/L) 6-BA(1mg/L)为宜.对于叶片丛生芽愈伤组织培养基用MS NAA(0.2mg/L) 6-BA(2.8mg/L),下胚轴丛生芽愈伤组织培养基用MS NAA(0.01mg/L) 6-BA(0.1mg/L);MS NAA(0.05mg/L) 6-BA(0.1mg/L)为宜,诱导生根以1/2MS NAA0.3mg/L最佳,生根率达95%以上.
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| 关 键 词： | 祁门白术 组织培养 植株再生 |
| 文章编号： | 1672-447X(2006)05-0057-04 |
| 修稿时间： | 2006-06-20 |
Study on Atractylodes macroephala Koidz By Tissue Culture and Rapid Propagation |
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| Hu Changyu,Ye Yujuan,Hao Xiangxia,Hu Yue. Study on Atractylodes macroephala Koidz By Tissue Culture and Rapid Propagation[J]. Journal of Huangshan University, 2006, 8(5): 57-60 |
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| Authors: | Hu Changyu Ye Yujuan Hao Xiangxia Hu Yue |
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| Abstract: | For the study on the rapid propagation of Atractylodes macroephala Koidz,the aseptic inferior embryo spindles and leaves were used as explants cultured in solid medium MS supplemented with NAA,6-BA or combinations of plant growth regulators with different concentrations .The results showed that Better results could be obtained by the inferior Embryo spindle; the optimum combination was MS NAA(0.5mg/L) 6-BA(2mg/L) for the leaves, MS NAA(0.5mg/L) 6-BA(2mg/L) for the inferior embryo spindle; for bud increasing, the leaf is MS NAA(0.2mg/L) 6-BA(2.8mg/L),and the inferior embryo spindle is MS NAA(0.01mg/L) 6-BA(0.1mg/L);for the increasing of root, we should use 1/2 MS NAA(0.5mg/L) ,and the effect can reach more than 95%. |
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| Keywords: | Atractylodes macroephala Koidz tissue culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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