Comparison in effect of different metal ions,pH and reducing agent on the protease activity in human hyper mature and mature cataract |
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Authors: | Sami Amtul Jamil Sami Amtul Naseer Kanwal Noreen |
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Institution: | (1) Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, 54594, Pakistan;(2) Institute of Ophthalmology, King Edward Medical University, Lahore, 54594, Pakistan |
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Abstract: | This study was undertaken to isolate and characterize the protease activity of human eye lens sample of mature and hyper mature
cataract. Samples were collected just after surgery of the cataract lens and were stored at −20 °C. The total protein extract
was isolated from 5 samples in each case (mature and hyper mature cataract) and clear supernatant obtained after centrifugation
was used as an enzyme source. The optimum pH for the proteases of mature cataract was 7.5 while the proteases of hyper mature
cataract were recorded for maximum activity at pH 5.5 and 7.5. The optimum temperature for both enzyme sources was 50 °C.
Effect of different metal ions such as potassium, lead, silver, zinc and borate was studied. In each case protease activity
was increased. Reducing agent e.g. β mercapotethanol also caused an increase in activity indicating the involvement of sulfhydryl
groups. Protease activity was also located on agar plates.
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Keywords: | Mature cataract Hyper mature cataract Eye lens Protease activity |
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