| Receptor-mediated gene delivery using polyethylenimine （PEI） coupled with polypeptides targeting FGF receptors on cells surface |
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| 作者姓名： | Li D Wang QQ Tang GP Huang HL Shen FP Li JZ Yu H |
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| 作者单位： | [1]Institute of lmmunology, Zhejiang University, Hangzhou 310058, China [2]Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou 310028, China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Objective： To construct a novel kind of nonviral gene delivery vector based on polyethylenimine （PEI） conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods： The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides including DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results： The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR 16 could be blocked by excess free polypeptides. Conclusion： The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.
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| 关 键 词： | FGF受体 聚氮丙啶 目标命中 基因治疗 |
| 收稿时间： | 2006-02-21 |
| 修稿时间： | 2006-04-21 |
Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface |
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| Li D,Wang QQ,Tang GP,Huang HL,Shen FP,Li JZ,Yu H.Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface[J].Journal of Zhejiang University Science,2006,7(11):906-911. |
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| Authors: | Da Li Qing-qing Wang Gu-ping Tang Hong-liang Huang Fen-ping Shen Jing-zhong Li Hai Yu |
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| Institution: | (1) Institute of Immunology, Zhejiang University, Hangzhou, 310058, China;(2) Institute of Chemical Biology and Pharmaceutical Chemistry, Zhejiang University, Hangzhou, 310028, China |
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| Abstract: | Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (PEI) conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods: The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides in-cluding DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results: The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR16 could be blocked by excess free polypeptides. Conclusion: The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy. |
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| Keywords: | FGF receptors Polyethylenimine Targeting Gene delivery Gene therapy |
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