血浆纤维蛋白原含量升高与冠心病

用盐析－双缩脲法对９７例健康人和７９例冠心病患者进行了血浆纤维蛋白原含量的测定，结果表明：冠心病患者血浆纤维蛋白原含量显著高于健康人（Ｐ＜０．０１），提示纤维蛋白原含量升高是冠心病、中风、血栓形成的一种重要的危险因素．对血浆纤维蛋白原进行检测，将为冠心病的临床诊断及预防提供依据     

冠心病患者体外血栓和血小板聚集功能测定的初步探讨

对７１例健康人与８３例冠心病患者体外血栓、血小板聚集功能测定比较，结果证明冠心病患者的体外血栓和血小板聚集明显高于健康人（Ｐ＜０．０１）。     

冠心病患者血浆同型半胱氨酸变化及叶酸的干预治疗

目的 了解血浆同型半胱氨酸(Homocysteine，Hcy)、叶酸和维生素(Vit)B12水平与冠心病的关系；观察叶酸干预治疗高Hcy血症的疗效．方法 经冠脉造影确诊的冠心病患者97例、亚冠心病患者58例及完全正常3l例，用高效液相色谱法测血浆Hcy水平，用放免法测血浆叶酸和VitB12浓度．其中高Hcy血症病人每天服用叶酸5mg，观察4周和8周后血浆Hcy水平变化．结果 冠心病和亚冠心病患者血浆Hcy水平明显高于正常人，叶酸和VitB12水平则明显下降；冠心病患者冠脉病变支数越多，血浆Hcy升高和叶酸、VitB12下降越明显；叶酸治疗高Hcy血症患者4周和8周，分别使血浆Hcy水平下降12．5％和38．5％．结论 冠心病和亚冠心病患者血浆Hcy水平明显升高，高Hcy血症是冠心病的危险因素之一．血浆Hcy水平变化与冠脉病变程度正相关．叶酸干预治疗可使高Hcy血症患者血浆Hcy浓度明显降低．     

Hsp90在大肠癌患者血液中表达及意义

探讨Hsp90在大肠癌患者及正常人血清中的表达及意义。方法:通过对150例血浆样本,包括80例大肠癌患者、70例健康人采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)进行检测,测定Hsp90蛋白的血浆浓度,检验其诊断大肠癌的敏感性和特异性。结果:ELISA检测结果显示,大肠癌患者血浆中Hsp90蛋白浓度为(86.54±18.20)ng/m L,显著低于正常对照人群(240.18±52.72)ng/m L(P0.01)。当cutoff值定为124.29 ng/m L时,Hsp90检测大肠癌的敏感性为96.5%,特异性为87.8%。结论:检测血浆中Hsp90的蛋白水平有助于大肠癌的诊断。     

原发性高血压与高纤维蛋白原血症：（附68例分析）

本文测定了68例原发性高血压患者的血浆纤维蛋白原浓度,并与50例正常血压者作了对照,发现原发性高血压组的血浆纤维蛋白原浓度为700.3±211.4 mg/dl,比对照组高(P相似文献   

血浆纤维蛋白原水平升高同高血压,冠心病及卒中的联系

选择46岁以上的高血压(HBP)、冠心病(CHD)、卒中及其它内科住院病人共140名进行了血浆纤维蛋白原(FG)、血压、甘油三酯(TG)和吸烟等因素的观察.结果表明高血压、冠心病和车中患者FG值升高,逐步回归分析看出吸烟,HBP和TG水平升高明显影响FG的含量,提示FG水平升高同HBP,CHD和年中存在着紧密的联系。     

高脂血症病人血液流变学指标的测定

本文对９７例健康人与４４例高脂血症病人进行血液流变学等１０项指标的测定与比较，实验结果证明，高脂血症病人的全血粘度（２３０．００ｓ＾－１，２３．００ｓ＾－１，９．６０ｓ＾－１），血浆粘度（２３０．００ｓ＾－１），纤维蛋白原等均明显高于健康人，经统计学处理（Ｐ＜０．０１），有显著差异。     

脑脊液蛋白含量变化与吉兰巴雷综合征治疗方案的相关性研究

目的:用脑脊液蛋白含量变化作为吉兰巴雷综合征(Guillain-Barre syndrome,GBS)治疗效果的观察指标,评价治疗方案的有效性及安全性。方法:对63例广西医科大学第二附属医院住院的存在明显肌力障碍的GBS患者,用治疗前后2次脑脊液蛋白含量商作为治疗观察指标,作多因素相关分析,判断治疗方案的有效性及安全性。结果:年龄、前驱感染、血浆置换、激素冲击与蛋白商显著独立相关(P0.05),其中血浆置换呈正相关,对治疗有效;激素冲击呈负相关,提示可能对GBS患者不利。结论:脑脊液蛋白含量变化是GBS疗效评价的良好指标。     

老龄冠心病患者的核酸基与蛋白多肽分析及诊疗

目的:老龄冠心病患者体内的核酸基与蛋白多肽具有差异性特征,通过对该类核酸基与蛋白多肽差异性特征进行分析,了解并干预危险因素,进行冠心病预防,实现对老龄冠心病患者的有效诊疗。方法:以某专科医院收治的33例老龄冠心病患者为受试对象,对患者进行临床跟踪观察和生化指标测试,研究老龄冠心病患者体内的核酸基与蛋白多肽特征。对影响冠心病的TSH进行抑制,在高密度脂蛋白胆固醇聚合时,会对核酸基产生一定的影响,造影剂选用Annner公司生产的肺功能生理指标造影显示剂,采用Valsalva试验,利用Kinase-Glo Luminescent Kinase Assay Kit测定蛋白脂质体中磷脂和蛋白含量,采用SPSS12.0软件进行统计学分析,得到治疗方案。结果:用碳-碳三键进行系列转化,当向反应体系中加入自由基引发剂(AIBN,10 mol%),形成光学相干断层成像(OCT),再结合辅助检查发现心肌缺血或冠脉阻塞的证据,进行冠状动脉造影,进行老龄冠心病患者的统计分析及治疗。通过调节不同时刻蛋白多肽和和核酸基,身体逐步增加红细胞和血红蛋白的生成,改善冠心病患者的血液输氧的能力。结论:对老龄冠心病的检查方法包括常规心电图和心电图负荷试验、核素心肌显像,同时进行高危冠心病患者的筛查,采用该方法对稳定性冠心病和急性冠状动脉综合征具有显著的疗效和预防作用。     

伴与不伴高血压的慢性充血性心力衰竭患者血浆内皮素一氧化氮水平的比较及卡维地洛的干预研究

目的探讨血浆内皮素(ET)和一氧化氮(NO)在伴与不伴高血压的慢性充血性心力衰竭(CHF)患者中的变化及其临床意义。方法选取152例CHF患者,根据是否合并有原发性高血压分为伴高血压CHF组(72例)和不伴高血压CHF组(80例),两组按治疗方法不同又随机分为卡维地洛治疗亚组和常规治疗亚组,同时设健康体检人员30例为对照组。分别测定治疗前后血浆ET水平和NO水平,并进行统计学分析。结果伴高血压CHF组患者的ET水平和不伴高血压CHF组血浆ET及NO水平均明显高于正常对照组(P<0.05),各亚组治疗后均下降(P<0.05),以卡维地洛亚组下降更明显(P<0.01);伴高血压CHF组NO水平均明显低于正常对照组(P<0.05),两亚组治疗后NO均上升(P<0.05),以卡维地洛亚组上升更明显(P<0.01)。结论 ET和NO与高血压病和CHF的发生发展关系密切,卡维地洛可改善了CHF患者的神经内分泌状态及血管内皮系统的功能紊乱。     

Endothelins -- clinical perspectives

Endothelins (ET) are a group of endogenous peptides, which have a strong and long-lasting vasoconstrictive effect. Three isoforms of endothelins coded by three different genes have been identified to date. Endothelin-1 (ET-1) is the most potent vasoconstrictive agent currently identified, and it was originally isolated and characterized from the culture media of aortic endothelial cells. Two other isoforms, named endothelin-2 (ET-2) and endothelin-3 (ET-3), were subsequently identified, along with structural homologues isolated from the venom ofActractapis engaddensis known as the sarafotoxins. The biological effects of endothelin production are determined via activation of one or two G-protein coupled receptors, endothelin receptors A (ETRA) and B (ETRB1 and ETRB2). Recently endothelin receptor C (ETRC) was discovered, however, its functions and distribution still remain unclear. The effects mediated by ET-1 via ETRA are vasoconstriction, bronchoconstriction and secretion of aldosterone. Agonists related to the ETRB1 activation cause vasodilatation by stimulating NO, PGI2 and endothelium-derived hyperpolarizing factor (EDHF). In contrast, coupling to ETRB2 causes vasoconstriction. Involvement of ET has been demonstrated in the pathophysiology of certain disorders. In this review, we discuss the physiological and pathophysiological role of endothelium-derived ET-1, the pharmacology of its two receptors, focusing on the role of ET-1 in the development of some pathophysiological conditions.     

冠心病患者和正常人血脂与心率变异性频域指标关系的研究

目的：分析冠心病患者和正常人血脂水平与心率变异频域指标及自主神经活动的关系。方法：对71例确诊的冠心病患者和96例正常人行24h动态心电图并采空腹血查血脂，分别比较两类人群中高胆固醇血症组与正常胆固醇组心率变异频域指标VLF、LF、HF、LF／HF的差异，并用多元逐步回归分析血脂各值与心率变异的相关性。结果：高胆固醇血症组与正常胆固醇组相比，冠心病患者中HF值显著降低（P＜0．05），而LF／HF值显著增高（P＜0．01）；正常人亦有高胆固醇血症组HF值较低而F/HF值较高的趋势，但无统计学差异。多元逐步回归分析中，冠心病患者HF与TC呈负相关，LF／HF则与TC、LDL呈正相关；正常人中LF／HF亦与TC呈正相关。结论：冠心病患者和正常人中血脂水平均与心率变异及自主神经活动存在一定的相关性。胆固醇增高者交感与迷走神经平衡性失调，交感神经活性相对较高而迷走神经活性相对较低。     

Biochemical changes during exchange transfusion in hyperbilirubinemia in term newborn babies

Double volume (170 ml/kg body weight) exchange transfusion was done in 52 term infants in the first week of life for neonatal hyperbilirubinemia. The M:F ratio was 1.08:1 and 37 (71.1%) babies were of low birth weight. Causes of jaundice were hemolytic in 46.2% and non-hemolytic in 41.3% cases; in 13.5% babies no cause of jaundice could be found. After exchange transfusion a fall of 14.6% and 47.4% was observed in the hemoglobin and serum bilirubin levels respectively. There was significant (p=0.0414) rise in the mean mid exchange and post-exchange serum sodium levels as compared to pre-exchange values and it was found to be due to higher donor's serum sodium levels (p=0.007). There was no effect on the serum potassium levels during or after ET. In general serum calcium levels significantly increased at mid-exchange period (p=0.0029) but post-exchange levels were same as pre-exchange. Donor's serum calcium level had no effect on the infant's serum calcium level (p=0.993). There was no change in the serum phosphate and blood urea levels during and after exchange-transfusion. The plasma glucose was significantly raised during and after ET and plasma glucose of the donors had significant effect on the infant's plasma glucose levels (p=0.043). Similarly plasma osmolality also showed significant increase during and after ET which was due to the effect of donor's plasma osmolality (p=0.007).     

Inflammatory Cytokines,Apoptotic, Tissue Injury and Remodeling Biomarkers in Children with Congenital Heart Disease

The present study aims to evaluate specific biomarkers involved in congenital heart disease (CHD), and whether there is a significant differences between the levels of these biomarkers in the cyanotic CHD (CCHD) and acyanotic CHD (ACHD). We prospectively measured tumor necrosis factor (TNF-α), interleukin-6 (IL-6), C-reactive protein (CRP), vasoendothelial growth factor (VEGF), troponin T, creatin kinase MB (CKMB), and Caspase 3 levels in 120 consecutive children with CHD (60 cyanotic and 60 a cyanotic with age 1:4 years), and 30 healthy control children. Significant elevated levels of inflammatory markers; TNF-α, IL-6 and CRP was detected in CHD, with percentage increase in cyanotic than a cyanotic subjects as compared to the normal one. Apoptotic biomarker; caspase 3 showed also significant increases in CCHD than ACHD. In addition, tissue injury mechanisms included troponin T and CKMB, exhibited significant increase in cyanotic than a cyanotic CHD. The present results demonstrate also, significant enhancement in remodeling process (VEGF), in cyanotic than a cyanotic patients. Thus, it could be concluded that, the children with CCHD were shown to have elevated levels of inflammatory cytokines, caspase 3, troponin T, and CKMB as these biomarkers may implicated in cardiac functional status.     

汾沁地区蒸散模拟及其时空变化特征

蒸散是水循环和能量循环的重要过程,也是连接土壤-植被-大气系统的关键纽带。气候变化背景下,蒸散的时空分布研究可为地区水资源合理配置及应对气候变化提供科学基础。本文基于结合GRACE（Gravity Recovery and Climate Experiment）数据的水量平衡方法模拟黄土高原汾沁地区2003—2015年蒸散量,并分析其不同时间尺度的变化特征,结果表明：①结合水储量变化的水量平衡方法与忽略水储量变化的水量平衡方法模拟所得蒸散相比,前者时间序列上波动更平稳（变异系数、标准差、极端值分别减少0.12,5.50mm,3.20%）,可更精确地反映汾沁地区实际蒸散在年和季节尺度上的变化规律;②研究区2003—2015年均蒸散量为530.19mm,空间分布上由北向南大致增加,年际波动较平稳（变异系数为0.08）,其中2010年蒸散量最低（478.22mm）,2011年蒸散量最高（614.57mm）;③季节尺度上,夏季平均蒸散量最高（263.36mm）,占全年蒸散量的49.67%,波动较平稳;冬季蒸散量最低（19.50mm）,离散程度较大;④汾沁地区2003—2015年蒸散变化主要受温度、降水的影响,其年际波动主要与降水相关。     

汉江上游流域蒸散量计算方法的比较及改进

利用汉江上游流域13个气象站的气象观测数据,对SWAT2000模型内嵌的3种潜在蒸散量计算方法:①Penman-Monteith(PM)法、②Pristley-Tayler(PT)法和③Hargreaves法以及FAO Penman-Monteith(FAO PM)法、DeBruin法进行了蒸散量(ET0)计算方法的分析和评价.以气象站实测小型蒸发数据经过折算得到的陆面潜在蒸散量为标准,对模拟蒸散量结果进行检验,根据相关系数、解释方差、确定性系数以及相关曲线斜率等4个指标的比较,详细讨论了各方法的适用性及模拟精度,认为5种方法中PM法和FAO PM法模拟精度最高.但是进一步分析发现FAO PM法存在部分中间气象参量计算错误以及存在与实测数据偏差较大的情况,会影响到水文过程中其它子过程的模拟.因此选择PM法作为汉江上游流域蒸散量计算的首选方法,并通过数据分析提出利用高程对模拟结果进行修正,提高了蒸散量模拟的精度.     

Standardization of homocysteine on HPLC using DTT as reductant

Determination of plasma total homocysteine by high-pressure liquid chromatography (HPLC) usually requires reduction of protein bound or free homocysteine-disulphides into thiols by a reducing agent and the liberated thiols are then derivatized by a fluorescent marker. In this study we have standardized the HPLC method for homocysteine measurement using dithiothreitol (DTT) as reductant. The results of plasma total homocysteine values obtained by HPLC were compared with IMx method. The difference between the two means was statistically insignificant [P=0.616847 (two tail)] Linear regression analysis showed strong correlation between the two methods (r=0.983). Using this method we have analyzed 132 controls and 130 Coronary Heart Disease (CHD) patients for plasma total homocysteine, wherein, the mean plasma total homocysteine levels were 10.51±8.36 and 11.51±10.06 μmol/L respectively. Our research study suggests that DTT method is a simple and inexpensive assay for homocysteine determination in human plasma for research application.     

三种高分辨率地表蒸散发产品在华北地区的验证与对比

华北平原是中国重要的农业主产区,同时也是世界上水资源短缺最为严峻的地区之一。地表蒸散发（ET）是水资源消耗的最大项,因此获取准确的ET数据是华北平原水资源管理的重要基础。本文对全球3种高分辨率ET产品在华北地区进行精度验证和时空对比,以期为选择更适用于华北平原的高分辨率ET数据提供参考信息,更好地为水资源的研究和管理服务。通过与涡动相关测量数据对比,研究显示PML_V2产品在华北地区精度最高,其次是SSEBop_V4,最后是MOD16A2,相关系数分别为0.81、0.74和0.52;均方根误差分别为0.87、1.52和1.44 mm/d,PML_V2与站点观测值的波动趋势一致性最高。3种产品在小麦生长季的估算值与观测值的相关性均高于玉米生长季,SSEBop_V4和PML_V2估算值分别在小麦季和玉米季与观测值具有最高的相关性。通过3种产品相互之间的对比,结果发现PML_V2和SSEBop_V4在空间分布上较一致,相关系数最高,为0.76;MOD16A2的空间分布与其他2个产品差异较大;三者的最大差异出现在耕地区。从2003—2018年的变化趋势上,MOD16A2在3种土地利用类型下明显呈增加趋势,而SSEBop_V4和PML_V2无明显变化。该研究结果有助于评估每种产品的质量和不确定性,以改进ET算法和产品质量。