奶牛临床型乳房炎的治疗方法

奶牛临床型乳房炎是影响奶牛产奶量的主要疾病之一,采用乳房送药、静脉注射、中药灌服治疗,效果良好。本文主要介绍了奶牛临床型乳房炎的发病原因、治疗方法和预防措施。     

Prevalence, genetic diversity, and antimicrobial susceptibility profiles of Staphylococcus aureus isolated from bovine mastitis in Zhejiang Province, China

This study was conducted to determine genetic diversity and antimicrobial susceptibility profiles of Staphylococcusaureus recovered from bovine mastitis in Zhejiang Province, China. Out of 3178 quarter milk samples from 846 lactating cows, among which 459 cows (54.3%) were found HMT positive, 890 quarters (28%) were found having subclinical mastitis. From 75 representative S. aureus isolates, 16 distinct types were identified by pulsed-field gel electrophoresis (PFGE). Four major PFGE types (A, B, C, and D) accounted for 82.7% of all isolates, and type A (41.3%) was observed in multiple herds across the studied areas. Each region was found to have a predominant type: Hangzhou type A (64.1%), Ningbo type C (34.5%) and type B (23.1%), Jinhua type D (53.3%), and Taizhou type C (62.5%). Results of antimicrobial susceptibility tests showed that 90.7% of the isolates were resistant to at least one antimicrobial. Resistance to penicillin and ampicillin (77.3%), tetracycline (60.0%), or erythromycin (48.0%) was observed. The bacteria resistant to multiple antibiotics such as penicillin, ampicillin, tetracycline, and erythromycin were commonly found. The information obtained from this study is useful for designing specific control programs for bovine S. aureus mastiffs in this region.     

Persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis

This study was designed to investigate persistence of gentamicin residues in milk after the intramammary treatment of lactating cows for mastitis. Milk samples were collected at a 1-d interval after the last administration from 34 individual cows that had received intramammary infusions of gentamicin. The doses and treatment times evaluated in this study represented those that have been applied by veterinarians in practice. The tetrazolium chloride assay was used to determine whether there were sig-nificant residues of the antibiotic in the samples. Persistence of detectable drug residues in milk from 33 cows (28 cows, <6 infusions at≤0.7 g gentamicin; and 5 cows, 2 infusions at 0.8 g gentamicin) did not exceed 5 d; but 1 cow (5 infusions at 0.8 g gentamicin) had detectable residues in its milk for 9 d. Our results suggest that a 5-d milk withdrawal period might be insufficient to secure the clearance of the contamination of gentamicin, because treatment times and dosages contribute to the antibiotic clearance. A larger scale of samples are needed for further investigations.     

壳聚糖对乳房炎奶牛血清胆固醇、尿酸和肌酐的影响

为确定壳聚糖对奶牛乳房炎的治疗效果,选择产奶量、胎次和泌乳期相近,营养中等,乳房炎阴性奶牛6头,阳性30头,组成0(阴性对照组)、1(阳性对照组)和2、3、4、5组(试验组),每组6头.各组基础日粮和饲养管理相同.分别在基础日粮中添加壳聚糖0g、0g、10g、20g、40g和80g/d/头.连续喂养2周,分别与试验的0d、7d、14d采取血样,分离血清,测定血清中胆固醇、尿酸和肌酐的含量.结果:应用壳聚糖7～14d后,2、3、4、5组奶牛血清胆固醇含量均数有随壳聚糖用量的增加而降低的趋势,但差异不显著(P﹥0.05);血清尿酸和肌酐水平显著(P﹤0.05)或极显著(P﹤0.01)地降低.结论:壳聚糖具有加快乳房炎患牛机体对尿酸和肌酐排泄的功能,提高机体对炎症的抵抗力,促进机体的康复过程.     

Development and evaluation of immunoassay for zeranol in bovine urine

A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine.     

选择遗传标记性状降低奶牛隐性乳房炎发生率的设想

设想通过后裔测定选择较低体细胞数 (SCC)估计育种值的公牛 ,以降低奶牛隐性乳房炎的发生率 ;对公牛进行包括生产性能、体型、SCC等各个方面的综合、全面的评价 ;考虑平衡地、较充分地发挥公牛降低隐性乳房炎发生率的遗传改良作用     

饲喂生物活性蛋白粉对奶牛生产性能的影响

用生活活性蛋白粉饲喂奶牛,研究其对奶牛生产性能的影响.采用逐头配对法,选取38对产奶牛分为试验组与对照组,试验组奶牛精饲料添加4%的生物活性蛋白粉,进行为期38d的试验检测,并用t检验对各组试验前后指标进行比较,结果表明试验组与对照组的产奶量差异显著(p<0.05),乳脂率差异不显著(p>0.05).饲喂生物活性蛋白粉可显著提高产奶量,并可获得明显的经济效益.     

A corn straw-based diet increases release of inflammatory cytokines in peripheral blood mononuclear cells of dairy cows

Recent studies have shown that diet can affect the body’s immunity. Roughage of dairy cows consists of a variety of plant materials which make different contributions to health. This study investigated the effect of different roughages on the immunity of dairy cows. Serum, peripheral blood mononuclear cells (PBMCs), and milk samples were collected from 20 multiparous mid-lactation cows fed mixed forage (MF)- or corn straw (CS)-based diets. Expression profile analysis was used to detect the differentially expressed genes (DEGs) from PBMCs. The results showed that milk protein in the MF group increased to 3.22 g/100 ml, while that of the CS group milk was 2.96 g/100 ml; by RNA sequencing, it was found that 1615 genes were differentially expressed between the CS group and the MF group among the 24 027 analyzed probes. Gene ontology (GO) and pathway analysis of DEGs suggested that these genes (especially genes coding cytokines, chemokine and its receptors) are involved in the immune response. Results were confirmed at the protein level via detecting the levels of interleukin-2 (IL-2), IL-6, IL-10, IL-12, leptin (LEP), interferon-γ (IFN-γ), transforming growth factor-β1 (TGF-β1), and tumor necrosis factor-α (TNF-α) in peripheral blood by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay analysis. Our data supported the conclusions that the protein content in milk of the MF group was higher than that of the CS group, the CS-based diets induced more release of cytokines than the MF-based diets in dairy cows’ PBMCs, and milk protein content may be affected by cytokines.     

Acupuncture for treating polycystic ovary syndrome: guidance for future randomized controlled trials

Objective

To provide guidance for future randomized controlled trials (RCTs) based on a review concerning acupuncture for treating polycystic ovary syndrome (PCOS).

Methods

A comprehensive literature search was conducted in October 2015 using MEDLINE, EMBASE, SCISEARCH, Cumulative Index to Nursing and Allied Health Literature, the Cochrane Menstrual Disorders and Subfertility Group trials register, Allied and Complementary Medicine (AMED), China National Knowledge Infrastructure (CNKI), and the Wanfang databases. RCTs comparing either acupuncture with no/sham/pharmacological intervention or a combination of acupuncture and conventional therapy with conventional therapy in the treatment of PCOS were included in this review. A quality evaluation was performed for each of the included studies.

Results

Thirty-one RCTs were included in the review and were divided into four categories according to the type of intervention used in the comparator or control group. Menstrual frequency, hormones, anthropometrics, insulin sensitivity, blood lipids, and fertility were used as the main measurements to assess the effects of acupuncture on the patients with PCOS. Thirty trials, except for one, showed an improvement in at least one of the indicators of PCOS after acupuncture treatment. However, normalizing the methodological and reporting format remains an issue.

Conclusions

Based upon this review of current clinical trials concerning acupuncture for treating PCOS, we provide guidelines for better clinical trial design in the future.

     

针刺配合推拿治疗急性乳腺炎58例疗效观察

目的:观察针推治疗急性乳腺炎的疗效.方法:采用患野与远端辨证取穴相结合,运用针刺配合推拿进行治疗.结果:58例患者痊愈49例,显效7例,无效2例,总有效率96.5%.结论:针刺配合推拿治疗本病效果满意.     

猪圆环病毒Ⅱ型河南地方株ORF4的克隆及原核表达

根据GenBank数据库中猪圆环病毒Ⅱ型的基因组序列设计引物,采用PCR技术从病料基因组DNA中扩增出PCVⅡ河南地方株ORF4基因,全长180 bp,编码59个氨基酸.将该基因克隆至载体pGEX-4T-3中形成pGEX-4T-3-ORF4表达载体.经PCR、酶切和测序鉴定后,转化表达菌株BL21（DE3）诱导表达.SDS-PAGE结果显示：ORF4能够在大肠杆菌中表达,产物的分子量约为32 kD,且以包涵体形式存在.Western Blot检测结果显示,纯化后的ORF4蛋白能够与鼠抗6＆#215;His标签单克隆抗体发生特异性反应,为进一步研究PCVⅡORF4蛋白的特性与功能奠定了基础.     

The Role of Agricultural Education and Extension in Influencing Best Practice for Managing Mastitis in Dairy Cattle

Purpose: To examine the role of agricultural education and extension in influencing the adoption of best practice with regard to herd-level mastitis management.Design/Methodology/Approach: Somatic cell count (SCC) is an indicator of herd health with regard to mastitis and is negatively related to productivity and profitability. Panel data regression methods are utilised here to quantify the role of agricultural education and extension in reducing cell count and in influencing farmer best practice with regard to herd health. The impact of education and extension on farmer uptake of milk recording is of particular interest. Data utilised is farm-level Farm Accountancy Data Network (FADN) data for Ireland over a five-year period (2008–2012).Findings: Farmer uptake of formal agricultural training and liaison with agricultural extension services are shown to significantly improve mastitis herd health. Collectively, education, extension and milk recording results in an overall SCC reduction of 25% for the average herd. Farmers who undertook agricultural training were ten times more likely to monitor milk quality through milk recording compared to those who had not. Additionally, those farmers in contact with an extension service and also participated in a dairy discussion group were seven times more likely to practice milk recording.Practical Implications: The importance of farmer behaviour in the optimum management of herd health has been validated, as has the role of agricultural education and extension in influencing the uptake of best practice by farmers.Originality/Value: To date little research has been conducted using nationally representative herd-level data on the role of agricultural education and extension in improving animal health best practice.     

Clinicobiochemical investigations of gangrenous mastitis in does: immunological responses and oxidative stress biomarkers

A total of 50 does were used to determine selected hematological and biochemical parameters with special references to oxidative stress markers,acute phase protein profiles,and proinflammatory cytokines in healthy and gangrenous mastitis affected does.Animals were divided into two equal groups represented as clinically healthy(control) and diseased groups,respectively.The bacteriological examination of milk samples from diseased does revealed many types of bacterial infection.The isolated bacteria were Staphylococcus aureus(N=23/25),Escherichia coli(N=11/25),and Clostridium perfringens(N=4/25).There was a significant increase in the levels of β-hydroxybutyrate,non-esterified free fatty acids,triglyceride,low density lipoprotein cholesterol(LDL-C),aspartate aminotransferase,and alanine aminotransferase and a significant reduction in the levels of glucose,cholesterol,and high density lipoprotein cholesterol(HDL-C) in does with gangrenous mastitis compared to healthy does.Moreover,there was a significant increase in the levels of malondialdehyde and uric acid with a significant decrease in the levels of reduced glutathione,super oxide dismutase,and catalase in does with gangrenous mastitis compared to healthy does.In addition,there was a significant increase in the haptoglobin,serum amyloid A,fibrinogen,interleukin 6(IL-6),IL-1β,and tumor necrosis factor-α(TNF-α) in does with gangrenous mastitis compared to healthy ones.Conclusively,oxidative stress biomarkers,acute phase proteins,and proinflammatory cytokines play an essential task as biomarkers for gangrenous mastitis in does.Mastitis may be considered as one of the ketotic stressors in does after parturition.     

试论金华奶牛胚胎移植应用前景

金华奶牛发展迅速，数量持续上升。但高产良种比重偏少，泌乳母牛305d校正产奶量只有5000kg-5500kg左右。若要加快良种培育与推广，以期在尽可能短的时间内达到提高牛群总体质量的目的，那就必须建立金华奶牛良种繁育基地，大规模地应用胚胎移植技术。其前景将是十分广阔和诱人的。     

Iron determination after on-line sorbent preconcentration by fi-fass using 1, 10-phenanthroline as a complexing agent

1, 10-Phenanthroline (phen) was tested as a complexing agent for on-line preconcentration of iron on RP-C₁₈ material in a microcolumn with flow injection coupled with flame atomic absorption spectrometer (FI-FAAS). The on-line formed Fe(II)-phen complexes were adsorbed on the C₁₈ sorbent material. Various parameters affecting the on-line Fe(II)-phen complex formation and its subsequent adsorption in the microcolumn as well as its elution into the nebulizer of AAS were optimized. A 2.5×10⁻³ mol/L phen in 2% through the microcolumn for 30 s. The adsorbed Fe(II)-phen complexes in the microcolumn were eluted with ethanol in 10 s into the nebulizer of AAS. Ascorbic acid (5×10⁻⁴ mol/L) was added to the sample solution for the reduction of Fe(III) to Fe(II). A good precision (RSD=1.1%,n=10), high enrichment factor (19) and sample throughput (90 h⁻¹) with detection limit (3σ) of 3 μg/L were obtained. The method was applied on standard reference materials (i. e. mussel and tomato leaves,) for iron determination and yielded results agreeing well with certified values. Project supported by the Zhejiang Provincial Natural Science Foundation of China and Zhejiang Environmental Protection Foundation From Pakistan Institute of Nuclear Science & Technology, Islamabad, Pakistan and now ZJ University, Ph. D. student.     

When Your Breasts Might Not Work: Anticipatory Guidance for Health-Care Professionals

There are many factors that can negatively impact a mother developing a copious milk supply and being able to exclusively breastfeed her infant. In this article, we present two case exemplars (glandular hypoplasia and breast reduction surgery) to illustrate that not all mothers may be able to develop a full milk supply, and that families should receive appropriate prenatal anticipatory education and guidance from childbirth educators and all health-care providers. Important considerations include the value of every drop of milk that the mother is able to produce, treating the milk as an important medical intervention, and developing a plan with the family for supplementation so the infant can receive adequate intake for growth.     

Clock generator and OOK modulator for RFID application

The clock generator and OOK modulator for RFID (Radio Frequency Identification) presented in this paper consist of a current source and delay elements. The simple constant-gm structure is adopted in the current source design and the current consumption of the current source is only about 2 μA. The delay elements, the clock generator and OOK modulator are introduced in detail in the paper. The designed circuits are fabricated by 0.6 μm CMOS process. The area of the core circuit is only about 400 μmμ0 μm. The delay time of all three samples is in the range of 9 μs to 21 μs when the supply voltage varies from 2 V to 4 V. As the measured results satisfy the system requirements, these circuit structures are suitable for RFID application.     

免疫初乳对正常和实验性糖尿病小鼠血糖的影响

目的研究免疫初乳对正常小鼠和四氧嘧啶致糖尿病小鼠血糖的影响.方法用正常小鼠和糖尿病模型小鼠研究免疫初乳调节血糖的作用.连续30d给予正常小鼠和四氧嘧啶糖尿病模型小鼠免疫初乳,30d后眼眶取血,取其血清测定小鼠空腹血清血糖.结论免疫初乳能显著降低四氧嘧啶致高血糖小鼠的血糖水平并提高其糖耐量,且高剂量免疫初乳组的效果好于低剂量组的效果.但免疫初乳对正常小鼠的血清血糖及糖耐量没有影响.     

Determination of risperidone in human plasma by HPLC-MS/MS and its application to a pharmacokinetic study in Chinese volunteers

This study presents a rapid, specific and sensitive liquid chromatography/tandem mass spectrometry (LC-MS/MS) assay for determination of risperidone (RIS) in human serum using paroxetine as an internal standard (IS). An Alltima-C 18separation. The analysis was performed by selected reaction monitoring (SRM) method, and the peak area of the m/z 411.3→ 191.1 transition for RIS was measured versus that of the m/z 330.1→192.1 transition for IS to generate the standard curves. The assay linearity of RIS was confirmed over the range 0.25～50.00 ng/ml and the limit of quantitation was 0.05 ng/ml. The linear range corresponds well with the serum concentrations of the analytes obtained in clinical pharmacokinetic studies. Intraday and interday relative standard deviations were 1.85%～9.09% and 1.56%～4.38%, respectively. The recovery of RIS from serum was in the range of 70.20%～84.50%. The method was successfully applied to investigate the bioequivalence between two kinds of tablets (test versus reference products) in 18 healthy male Chinese volunteers. The result suggests that two formulations are bioequivalent.     

Large eddy simulation of a particle-laden turbulent plane jet

Gas-solid two-phase turbulent plane jet is applied to many natural situations and in engineering systems. To predict the particle dispersion in the gas jet is of great importance in industrial applications and in the designing of engineering systems. A large eddy simulation of the two-phase plane jet was conducted to investigate the particle dispersion patterns. The particles with Stokes numbers equal to 0.0028, 0.3, 2.5, 28 (corresponding to particle diameter 1 μm, 10μm, 30μm, 100μm, respectively) inRe=11 300 gas flow were studied. The simulation results of gas phase motion agreed well with previous experimental results. And the simulation results of the solid particles motion showed that particles with different Stokes number have different spatial dispersion; and that particles with intermediate Stokes number have the largest dispersion ratio. Project (No. G19990222) supported by the Special Funds for Major State Basic Research of China